两种仪器在微生物室间质评中的应用与比较

目的通过VITEK-32和Microscan AS-4微生物鉴定系统对室间质控菌株的鉴定,了解两种仪器的差异性及与质评结果的符合程度。方法对39株不同批次的质控菌株按操作规程进行分离与培养,用VITEK-32和Microscan AS-4进行鉴定并作比较。结果VITEK-32和Microscan AS-4能直接鉴定到种的菌株与39株质控株的符合率分别为76.9%和69.5%,经血清学或进一步生化验证后符合率分别为89.7%和76.9%,其中,二者对22株革兰阴性菌的鉴定符合率分别为90.9%和81.8%,对17株革兰阳性菌的鉴定符合率则分别为88.2%和58.8%。结论VITEK-32对微生物的检出率和准确性均优于Microscan AS-4,尤其在肠球菌属和链球菌属鉴定方面;二者对革兰阴性菌的鉴定准确性较好,对革兰阳性球菌的鉴定准确性稍差,而对需要血清学证实的沙门菌属、志贺菌属都不能鉴定到种。

VITEK-32 and Microscan AS-4 in External Quality Assessment of Microbes:Their Application and Comparison

OBJECTIVE By using the VITEK32 and Microscan AS-4 microbiology identification system to identify the external quality control(QC) strains of microbes,to find out the difference and the coincidence degree with quality assessment result of the two systems. METHODS Thirty nine various strains of QC bacteria were isolated and cultured according to operation rule,and were identified by VITEK-32 and Microscan AS-4 and compared within them. RESULTS The coincidence rate of VITEK-32 and Microscan AS-4 to identify directly their species was 76.9% and 69.5% for 39 strains of QC bacteria,It was 89.7% and 76.9% after the serum or further tests,and the coincidence rate was 90.9% and 81.8% in 22 strains of Gram negatives,and 88.2% and 58.8% in 17 strains of Gram positives. CONCLUSIONS The detectable rate and coincidence rate of VITEK-32 are superior to those of Microscan AS-4,especially in the identification of Enterococcus and Streptococcus,the accuracy of Gram negatives is good,and of Gram positives is short,both them could not identify Salmonella and Shigella to their species.