| Effects of Genistein on Proliferation and Cell Cycle of Salivary Adenoid Cystic Carcinoma Cells |
| |
| 引用本文： | 马杰,王洁,钟鸣,王兆元. Effects of Genistein on Proliferation and Cell Cycle of Salivary Adenoid Cystic Carcinoma Cells[J]. 中国癌症研究, 2007, 19(1): 64-67. DOI: 10.1007/s11670-007-0064-y |
| |
| 作者姓名： | 马杰 王洁 钟鸣 王兆元 |
| |
| 作者单位： | [1]Department of Oral Medicine, the Second Affiliated Hospital, China Medical University, Shenyang 110004 [2]Department of Pathology, College of Stomatology, China Medical University, Shenyang 110002 |
| |
| 摘 要： | Objective： To investigate the growth inhibiting effect of tyrosine protein kinase inhibitor, genistein, on human salivary adenoid cystic carcinoma SACC-83 cell line in vitro, and its effects on the expression of CyclinB1 protein and cell cycle. Methods： Effects of genistein on the growth of SACC-83 cells in vitro were measured with MTr assay. Cell cycle was detected with flow cytometry. The expressions of CyclinB1 and Cdkl proteins were measured with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. The results were statistically analyzed by SPSSI 1.5 software. Results： Genistein inhibited the cell proliferation in a dose-dependant and time-dependant manner. The genistein-treated SACC-83 cells were arrested in the G2/M phase and had lower contents of CyclinB1 and Cdkl proteins compared with the control group. Conclusion： The growth inhibiting effect of genistein on SACC-83 cells may be associated with the regulations of genistein on the CyclinB1 and Cdk1 protein expressions and the cell cycle.
|
| 关 键 词： | 唾液腺腺样囊性癌 癌细胞 染料木黄酮 细胞增长 细胞周期 |
| 文章编号： | 1000-9604（2007）01-0064-04 |
| 收稿时间： | 2006-04-27 |
| 修稿时间： | 2007-01-28 |
Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells |
| |
| Ma Jie,Wang Jie,Zhong Ming,Wang Zhao-yuan. Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells[J]. Chinese Journal of Cancer Research, 2007, 19(1): 64-67. DOI: 10.1007/s11670-007-0064-y |
| |
| Authors: | Ma Jie Wang Jie Zhong Ming Wang Zhao-yuan |
| |
| Affiliation: | (1) Department of Oral Medicine, the Second Affiliated HospitaL, China Medical University, Shenyang, 110004, China;(2) Department of Pathology, College of Stomatology, China Medical University, Shenyang, 110002, China |
| |
| Abstract: | Objective: To investigate the growth inhibiting effect of tyrosine protein kinase inhibitor, genistein, on human salivary adenoid cystic carcinoma SACC-83 cell line in vitro, and its effects on the expression of CyclinB1 protein and cell cycle. Methods: Effects of genistein on the growth of SACC-83 cells in vitro were measured with MTT assay. Cell cycle was detected with flow cytometry. The expressions of CyclinB1 and Cdk1 proteins were measured with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. The results were statistically analyzed by SPSS11.5 software. Results: Genistein inhibited the cell proliferation in a dose-dependant and time-dependant manner. The genistein-treated SACC-83 cells were arrested in the G2/M phase and had lower contents of CyclinB1 and Cdk1 proteins compared with the control group. Conclusion: The growth inhibiting effect of genistein on SACC-83 cells may be associated with the regulations of genistein on the CyclinB1 and Cdk1 protein expressions and the cell cycle. |
| |
| Keywords: | Adenoid cystic carcinoma Genistein CyclinB1 Cell cycle |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
|
