| 槲皮素和山柰酚逆转白血病细胞系K562/A02多药耐药性的机制及相关基因表达谱的研究 |
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| 引用本文： | 曹林娟,韩艳秋,郝洪军,石永进,徐国宾,任汉云.槲皮素和山柰酚逆转白血病细胞系K562/A02多药耐药性的机制及相关基因表达谱的研究[J].中国药学杂志,2011(11). |
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| 作者姓名： | 曹林娟 韩艳秋 郝洪军 石永进 徐国宾 任汉云 |
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| 作者单位： | 内蒙古医学院附属医院血液科;北京大学第一医院神经免疫室;北京大学第一医院血液科;北京大学第一医院检验科; |
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| 基金项目： | 内蒙古自然科学基金资助项目(200711020917); 教育部春晖计划科研合作项目(Z2007-1-01006) |
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| 摘 要： | 目的研究槲皮素(quercetin,Que)及山柰酚(kaempferol,Kae)单用及联合应用时对K562/A02细胞系多药耐药的影响及机制。方法体外培养K562和K562/A02细胞经Que及Kae处理,采用噻唑蓝(MTT)法计算药物单独及联合作用后细胞的生长抑制率及对多柔比星(adriamycin,ADM)的增敏倍数;流式细胞术检测药物作用后细胞内ADM浓度的变化;Annexin V/PI法观察细胞凋亡情况;Real-time PCR基因芯片检测药物转运蛋白及凋亡相关基因表达的变化。结果药物作用48 h后,Que及Kae在5～160μmol.L-1时对K562和K562/A02细胞均有剂量依赖性的生长抑制作用,K562/A02对ADM的敏感性显著增强,二者联用效果有协同性;在ADM为5μmol.L-1时,药物与细胞共培养2 h即可检出Que能增加细胞内ADM的浓度,而Kae则对ADM浓度无明显影响,两药联合作用的效果不及Que单独作用。Que和Kae均可剂量依赖性地诱导K562和K562/A02细胞的凋亡。二者可影响ABC、SLC家族等药物转运蛋白相关基因的表达,并可调节Bcl-2、TNF等凋亡相关基...
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| 关 键 词： | 槲皮素 山柰酚 K562/A02 多药耐药性 凋亡 |
Study on Mechamism of Reversing Multidrug Resistance by Quercetin and Keampferol and Their Effects on the Expression of Related Genes in Human Erythroleukemic K562/A02 Cells |
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| CAO Lin-juan,HAN Yan-qiu,HAO Hong-juna,SHI Yong-jinb,XU Guo-binc,REN Han-yunb.Study on Mechamism of Reversing Multidrug Resistance by Quercetin and Keampferol and Their Effects on the Expression of Related Genes in Human Erythroleukemic K562/A02 Cells[J].Chinese Pharmaceutical Journal,2011(11). |
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| Authors: | CAO Lin-juan HAN Yan-qiu HAO Hong-juna SHI Yong-jinb XU Guo-binc REN Han-yunb |
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| Institution: | CAO Lin-juan1,HAN Yan-qiu1,HAO Hong-jun2a,SHI Yong-jin2b,XU Guo-bin2c,REN Han-yun2b(1.Department of Hematology,Affiliated Hospital of Innermongolian Medical College,Huhhot 010050,China,2a.Department of Hematology,2b.Department of Neurology,2c.Department of Clinical Laboratory,Peking University First Hospital,Beijing 100034,China) |
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| Abstract: | OBJECTIVE To investigate the possibility and mechanism of quercetin and kaempferol,flavonoid molecules,for reversing multidrug resistance(MDR) in K562/A02 cells.METHODS K562 and K562/A02 cells were cultured in vitro with the flavonoids as single and in combination respectively.Cell growth inhibition and adriamycin(ADM) sensitivity were detected by MTT assay.Intracellular ADM concentration was determined by flow cytometry when K562/A02 cells was cultured with ADM and the flavonoids for 2 h.Cell apoptosis was... |
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| Keywords: | quercetin kempferol K562/A02 Multidrug resistance apoptosis |
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