SAG3基因在造血干细胞移植患者弓形虫感染诊断中的应用

目的探讨造血干细胞移植患者弓形虫感染的快速、特异、敏感的诊断方法。方法以弓形虫SAG3基因为靶基因，采用聚合酶链反应法(PCR)对56例造血干细胞移植受者及20名正常对照者进行检测，同时以酶联免疫吸附试验(ELISA)进行弓形虫抗体IgG、IgM的血清学检测。结果以PCR和ELISA检测弓形虫SAG3基因片段和其抗原，阳性分别为10例及7例，阳性率分别为17．9％和12．5％，其中PCR检测阳性、而ELISA检测阴性的3例，经病原学检测，均证实有弓形虫感染；20名正常对照者的检测结果均为阴性。结论体外扩增弓形虫SAG3基因的方法诊断弓形虫感染具有准确、快速、特异和敏感的优点，可作为造血干细胞移植患者弓形虫感染的诊断方法之一。

Amplification of SAG3 gene fragment and its application in detection of toxoplasma gondii in hematopoietic stem cell transplantation

Objective To establish a rapid, specific and sensitive diagnostic technique for the human T. gondii infection with hematopoietic stem cell transplantation and discuss its clinical significance. Me- thods Fifty-six patients subject to hematopoietic stem cell transplantation were detected with ELISA and PCR. Results Among 56 recipients of hematopoietic stem cell transplantation, 7 were positive for T. gondii antigen and 10 were positive for SAG3 gene fragment respectively with the positive rate being 14.3 % and 17.8 % in the ELISA and PCR screening respectively. Twenty healthy people were negative for anti-Toxo antibody.Conclusion PCR is an accurate, relatively rapid, sensitive and specific method for detecting SAG3 gene of T. gondii, and can be considered a valuable additional tool for identification of T. gondii infections.