| 实时荧光定量PCR法检测肺腺癌患者恶性胸腔积液中EMIA—ALK融合基因突变的研究 |
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| 引用本文： | 王逸飞,;于忠和.实时荧光定量PCR法检测肺腺癌患者恶性胸腔积液中EMIA—ALK融合基因突变的研究[J].临床肺科杂志,2014(7):1286-1288. |
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| 作者姓名： | 王逸飞 ;于忠和 |
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| 作者单位： | [1]安徽医科大学北京军区总医院临床学院,北京100700; [2]解放军北京军区总医院,北京100700 |
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| 基金项目： | 国家自然科学基金资助项目（No81372489） |
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| 摘 要： | 目的：探讨应用实时荧光定量PCR（ FQ-PCR）方法检测原发性肺腺癌患者恶性胸腔积液中的EML4-ALK融合基因突变情况,并对指导克唑替尼治疗的可行性进行探讨。方法收集39例原发性肺腺癌患者的胸腔积液,使用FQ-PCR法检测EML4-ALK融合基因的阳性率。结果胸腔积液中EML4-ALK融合基因的阳性率为12.8%,克唑替尼治疗对于EML4-ALK融合基因的和无EML4-ALK融合基因的患者相比有较高的疾病控制率（80% vs 16.7%,P〈0.05）。结论对难以获得组织标本的肺癌患者可选用FQ-PCR法检测肺癌患者恶性胸腔积液中EML4-ALK以指导克唑替尼的应用。
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| 关 键 词： | 肺腺癌 EML4-ALK 胸腔积液 实时荧光定量PCR |
Research of real-time fluorescent PCR to test the expression of EMIA-ALK in pleural effusion fluid of patients with lung adenocarcinoma |
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| Institution: | WANG Yi-fei, YU Zhong-he( Department of Oncology, the Military General Hospital of Belting PLA Clinical School Affiliated to Anhui Medical University, Beifing 100700, China) |
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| Abstract: | Objective To explore the clinical significance of FQ-PCR to detect EML4-ALK fusion gene in pleural effusion fluid of patients with lung adenocarcinoma. Methods 39 cases of MPE were collected. The mutation of EML4-ALK fusion gene was detected with FQ-PCR. Results There were 5 cases （12. 8%） with EML4-ALK fu-sion gene. Lung adenocarcinoma with EML4-ALK fusion gene had a higher disease control rate than those without mutation （80% vs 16. 7%, P〈0. 05）. Conclusion It is helpful to use FQ-PCR to detect EML4-AL in pleural ef-fusion fluid for patients with lung adenocarcinoma who have lost the opportunity to detect the gene mutation. |
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| Keywords: | lung adenocarcinoma EMIA-ALK pleural fluid FQ-PCR |
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