南京市鼓楼区中老年居民幽门螺杆菌感染 现状及相关因素研究

摘要：目的　了解南京市鼓楼区中老年居民幽门螺杆菌（ＨＰ）感染现状及其危险因素，为制定正确有效的 预防措施提供依据。方法　２０１７年５～８月对南京市鼓楼区９３５例居民进行尿素１４Ｃ 呼气试验测定，分析其 感染率；同时应用调查问卷开展危险因素调查，采用单因素和多因素ｌｏｇｉｓｔｉｃ回归分析幽门螺杆菌相关危险 因素。结果　９３５例居民中３６６例（３９．１４％）ＨＰ阳性；单因素ｌｏｇｉｓｔｉｃ回归分析显示有消化道疾病史、消 化道不适、是否吃辛辣和方便食品与ＨＰ感染有关（犘＜０．０５）；多因素ｌｏｇｉｓｔｉｃ回归模型拟合发现消化道疾 病史与ＨＰ感染负相关，而吃辛辣和方便食品与ＨＰ感染正相关（犘＜０．０５）。结论　南京市鼓楼区居民幽 门螺杆菌感染率较全国水平低，但感染仍普遍存在，应加强对消化道疾病的筛查与治疗，大力宣传健康安 全的饮食习惯，积极预防幽门螺杆菌感染与传播。 关键词：中老年；幽门螺杆菌；危险因素；问卷调查；流行病学研究 中图分类号：Ｒ１８３．４　　文献标识码：Ａ　　文章编号：１００９ ６６３９ （２０１９）０１ ０００１ ０５

Effect of MAPK signaling pathway on CEM-6T cell apoptosis induced by traffic-related PM2.5

ObjectiveTo explore the effect of MAPK signaling pathway on CEM-6T cell apoptosis induced by traffic related PM2.5, and to provide experimental evidence for understanding the mechanism of immunotoxic ity. Methods CEM 6T cells were exposed to PM2.5 at the doses of 0, 20, 80 and 320 mg/L for 24h or 48h. Western Blot was used to assess the effect of PM2.5 on p ERK, p-JNK and p38 inside cells. Meanwhile, the antagonists SP600125 for p JNK and SB203580 for p38 were also applied for further verification of the effect. One-way ANOVA was used for data statistical analysis. Results The p-ERK protein expression in CEM 6T cells decreased along with an increased exposure doses and time frames to traffic-related PM2.5. The lowest p-ERK protein level was noticed in cells exposed to the highest dose of 320 mg/L PM2.5 which had a significant difference compared to saline control （P〈0.05） . While, the p JNK1, p-JNK2 and p38 protein levels elevated in cells along with the increased doses of PM2.5 exposure. When cells were exposed to320 mg/L PM2.5, the differences of the above three protein levels were statistically significant compared to saline control （P-~0.0,5） . However, the addition of antagonists of p-JNK and p38 prior to the exposure could block the increases of p JNK2 and p38 protein levels, especially at the dose of 320mg/L. Conclusion Traffic related PM2.5 may induce CEM-6T cells apoptosis through the MAPK signaling pathway.