L929细胞条件培养液减轻氧化应激引起的U937细胞DNA断裂损伤

目的揭示巨噬细胞集落刺激因子（Ｍ－ＣＳＦ）的抗动脉粥样硬化作用是否与其保护单核细胞免受过氧化损伤有关。材料与方法用富含Ｍ－ＣＳＦ的Ｌ９２９细胞条件培养液（Ｌ９２９－ＣＭ）作为Ｍ－ＣＳＦ来源,以凝胶电泳、ＤＮＡ断裂定量等方法观察了Ｍ－ＣＳＦ对叔丁基氢过氧化物（ｔｂＯＯＨ）诱导的Ｕ９３７细胞ＤＮＡ断裂损伤的影响。结果ｔｂＯＯＨ可以引起Ｕ９３７细胞的ＤＮＡ断裂损伤,且损伤随ｔｂＯＯＨ作用时间的延长而趋明显;而Ｌ９２９－ＣＭ可以减轻ｔｂＯＯＨ诱发的Ｕ９３７细胞ＤＮＡ断裂损伤。结论Ｍ－ＣＳＦ对单核细胞具有一定的保护作用,可减轻其氧化损伤。

L929 cell-conditioned medium alleviates tert-butyl hydroperoxide-induced DNA fragmenta-tion in U937 cells

Objective To investigate the possible relation of the anti-atherosclerosis efficacy of macrophage colony-stimulating factor (M-CSF) with its protection of monocytes from oxidative injury. Materials and Methods The effect of L929 cell-conditioned medium (L929-CM) containing M-CSF on U937 cells incubated with tert-butyl hydroperoxide (tbOOH) was Investigated by electrophoresis and DNA fragmentation determination methods. Results It was shown that tbOOH could induce DNA fragmentation in U937 cells, and the proportion of cells with DNA fragmentation increased as the incubation pened prolonged, L929-CM could alleviate DNA fragmentation injury to U937 cells caused by tbOOH. Conclusion M-CSF could protect monocytes from oxidative injury.