信号传导与转录活化因子3、血管内皮生长因子和Survivin在胃癌组织中的表达及其临床意义

目的 探讨信号传导与转录活化因子3(STAT3)、血管内皮生长因子(VEGF)和Survivin蛋白在胃癌组织中的表达情况以及三者与胃癌临床病理特征的关系.方法 用免疫组织化学方法检测53例行胃癌根治切除的胃癌组织和53例正常胃组织中STAT3、VEGF和Survivin蛋白表达情况,并且分析3种蛋白表达与胃癌生物学行为的关系.结果 在本组53例胃癌标本中,STAT3、VEGF和Survivin蛋白的阳性表达率分别为58%、62%、74%,明显高于正常胃组织(P<0.01).胃癌组织中STAT3与VEGF、Survivin蛋白的阳性表达呈正相关(r=0.608、0.451,P<0.01),STAT3、VEGF的表达与病灶大小、Larren分型、有无淋巴结转移、临床分期有关(P<0.05),Survivin的表达与Larren分型、有无淋巴结转移、临床分期有关(P<0.05).多因素生存分析显示STAT3表达、有无淋巴结转移是预后的独立影响因素(OR=4.328、12.094,P<0.05).结论 STAT3通过上调VEGF和Survivin的表达促进胃癌组织中新生血管的形成、抑制肿瘤凋亡;STAT3可以作为判断胃癌预后的临床指标.

Exspression of STAT3, VEGF and survivin in gastric carcinoma

Objective To evaluate the expression of STAT3, VEGF and Survivin in human gastric carcinoma and its clinicopathological significance. Methods The expression of STAT3, VEGF, survivin was determined by immunohistochemical staining of specimens from 53 cases undergoing radical gastrectomy and 53 cases of normal gastric mucous membranae. We evaluated the relationship between expression of these proteins and various clinicopothological factors. Results The expression rate of STAT3, VEGF and survivin in 53 gastric carcinoma tissues was 58%, 62% and 74%, respectively, which was significantly higher than those in the normal group(P <0. 01). STAT3 expression correlated with VEGF(r =0. 608 ,P <0. 01) ,survivin(r = 0. 451, P = 0. 001). Positive STAT3, VEGF staining was significantly associated with tumor size, Lauren's classification,lymph node metastasis and clinical staging(P < 0. 05). Survivin staining was significantly associated with Lauren's classification, lymph node metastasis and clinical staging(P <0. 05). Multivariate analysis revealed STAT3 expression and lymph node metastasis were independently prognostic factors of poor survival. Conclusion VEGF, survivin possibly regulated by STAT3 leads to tumor angiogenesis and anti-apoptosis. The expression of STAT3 is an independent prognostic factors in gastric carcinoma.