磷酸酶基因、核因子-KB p65、Survivin蛋白在子宫内膜样腺癌中的表达及其临床意义

目的 探讨磷酸酶基因(PTEN)、核因子(NF-KB p65)及凋亡抑制蛋白Survivin基因在子官内膜样腺癌中的表达及其在子宫内膜样腺癌的发生、发展中的作用.方法 采用SP免疫组织化学法榆测63例子宫内膜样腺癌、20例正常增生期子宫内膜组织中PTEN、NF-KB p65及Survivin蛋白的表达.结果 子宫内膜样腺癌与正常增生期子宫内膜组织中PTEN、NF-KB p65及Survivin蛋白表达的阳性率差异有统计学意义(P＜0.01).PTEN表达与子宫内膜样腺癌分化程度呈正相关(P＜0.001),与淋巴结转移及TNM分期旱负相关(P＜0.005),NF-KB p65的表达与子宫内膜样腺癌分化程度旱负相关(P＜0.05),与浸润深度、淋巴结转移及TNM分期呈正相关(P＜0.05).Survivin与子宫内膜样腺癌与浸润深度、淋巴结转移及TNM分期呈正相关(P＜0.05).结论 PTEN、NF-KB p65及Survivin在子宫内膜腺癌的发生、发展中起着不同程度的作用,联合检测PTEN、NF-KB p65及Survivin对研究子宫内膜腺癌的发生、发展、早期诊断及预后评估有一定的参考价值.

Expression of PTEN,NF-KB p65 and Survivin in endometrial carcinoma and its clinical significance

Objective To explore the expression of PTEN,NF-KB p65 and Survivin protein and the influence in the genesis and development of endometrial carcinoma(EC).Methods The expression of PTEN.NF-KB and Survivin in 63 cases of EC and 20 cases normal endometrial tissue specimens were detected by immunohistochemistry.Resuits There were obvious differences among the positive rates of PTEN.N-KB p65 and Survivin protein in EC compared with in normal endometrial tissue specimens (P＜0.001).The clinicopathological characteristics of endometrial carcinoma with PTEN,NF-KB p65 and Survivin were as follows:the expression of PTEN gene was positively correlated with the degree of histological differentiation(P＜0.001),and it was negatively correlated with lymph node metastasis and TNM (P＜0.005).There was inverse correlation between the expression of NF-KB p65 gene and the degree of histological differentiation(P＜0.05).It was positively correlated with lymph node metastasis,deep myometrial invasion and TNM in EC (P＜0.05).The expression of Survivin was positively correlated with the clinical stage and lymph node metastasis and deep myometrial invasion in EC(P＜0.05).Conclusion There are difierent extent action of PTEN.NF-KB p65 and Survivin significantly in genesis and development of EC.Detection of PrEN combined with NF-KB p65 and Survivin is valuable for early diagnosing and evaluating malignancy extent of EC.