Concurrent induction of T-cell activation and apoptosis of osteosarcoma cells by adenovirus-mediated B7-1/Fas chimeric gene transfer |
| |
Authors: | Tsuji Hideki Kawaguchi Satoshi Wada Takuro Nagoya Satoshi Inobe Manabu Yagita Hideo Okumura Ko Yamashita Toshihiko Uede Toshimitsu |
| |
Institution: | Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan. |
| |
Abstract: | To establish an effective B7-based gene therapy against osteosarcoma, we transferred B7-1/Fas chimeric gene adenovirally into poorly immunogenic osteosarcoma cells. We found that adenovirus-mediated rat B7-1/Fas gene transfer induced (i) expression of rat B7-1/Fas chimeric molecules in osteosarcoma cells, (ii) activation of murine T cells, (iii) apoptosis of murine osteosarcoma cells in the presence of anti-rat B7-1 mAb in vitro, and (iv) therapeutic effects more prominently than B7-1 gene transfer on the development of pulmonary metastasis and survival of mice. These findings collectively support the therapeutic value of adenovirus-mediated B7-1/Fas gene transfer on poorly immunogenic osteosarcoma, which is resistant to a treatment protocol using transduction of B7-1 alone. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|