中国福建尖吻蝮蛇蛇毒类凝酶止血作用的实验研究

目的：研究中国福建尖吻蝮蛇蛇毒类凝酶止血作用及其作用机制。方法：用断尾法测小鼠出血时间，观察止血剂量对家兔全血凝固时间及纤维蛋白原含量的影响。结果：蛇毒类凝酶在0．0001－0．0100kU/kg可使小鼠出血时间明显缩短，而当剂量增大到3．0000kU/kg时，则显著延长小鼠出血时间。其0．0004－0．0036kU/kg剂量能使家兔全血凝固时间明显缩短，降低纤维蛋白原的含量。结论：中国福建尖吻蝮蛇蛇毒类凝酶在小剂量时可作为止血药，它可能通过适度降低纤维蛋白原的含量，缩短出凝血时间而发挥其止血作用。

Effect of Snake Venom Thrombin-like Enzyme from Agristrodom Acutus in Fu jian China on Hemostatic Action and Its Mechanism

Objective:To investigate the effect of snake venom th rombin like enzyme from Agristrodom Acutus in Fujian China on hemostatic action and its mechanism. Methods:The effect of snake venom thrombin like enzyme on bleeding ti me in mice and whole blood coagulation time and fibrinogen level in rabbit were observed.Results:snake venom thrombin like enzyme markedly shortened bleedin g time in mice at the dose of 0.000 1 0.01 kU/kg. When the dose increased to 3 kU /kg, bleeding time in mice was prolonged. The whole blood coagulation time and fibrinogen level in rabbit were shortened and decreased at the dose of 0.000 4 0.003 6 kU/kg.Conclusion:snake venom thrombin like enzyme from Agristrodom Acutus i n Fujian China acts as a hemostatic drug at small dose. It maybe exerts its hemos t atic action by properly decreasing fibrinogen level and shortening bleeding time and whole blood coagulation time.