RP—HPLC法测定鲨芪康胶囊中硫酸软骨素含量

目的建立RP．HPLC法测定硫酸软骨素的含量。方法采用RP-HPLC法测定，使用C18柱（4．6×250mm，5μm），流动相为乙腈．2．28mmol·L^-1四甲基氯化铵水溶液（5：95），流速为0．6mL·min^-1，检测波长195nm。结果硫酸软骨素总量在0．08-0．4mg·mL^-1浓度范围内线性关系良好（r=0．9996），方法平均回收率95．52％，RSD％=1．43％。结论本方法准确、快捷、重复性。可作为该制剂中硫酸软骨素的含量测定方法。

Determination of chondroitin sulfate in the shaqikang capsules by RPHPLC

OBJECTIVE A new RP-HPLC method was established for the determination of chondroitin sulfate. METHODS Use the method of RP-HPLC, with chromatographic column C18 （4.6 × 250mm, 5μm）, The mobile phase was acetonitrile-2.28mmol·L^-1 Tetramethylammonium chloride solution （5：95）at the flow rate of 0.6 mL·min^-1, and the detection wavelength was 195nm. RESULTS The calibration curve was linear in the concentration range of 0.08-0.4mg·mL^-1 （ r = 0. 9996） . The average recovery was 95.52 %, with RSD 1.43. CONCLUSION This method is precise, rapid and accurate, reproductibitily, and can be used as in the preparation method for the determination of chondroitin sulfate.