咽喉鳞癌组织中增殖细胞核抗原和核仁组成区相关嗜银蛋白的表达

目的 :研究 4 3例喉癌和 18例下咽癌术后标本中增殖细胞核抗原 (PCNA)的表达并对核仁组成区相关嗜银蛋白 (AgNOR)计数 ,分析其与临床分期、病理分化、复发、转移、生存期之间的相关性 ,评估其对预后及监测患者病情的价值。方法 :采用免疫组化法及Crocker’sAgNOR染色技术定量观察 6 1例鳞状细胞癌术后标本及9例良性喉肿瘤患者的非瘤区粘膜中PCNA的表达及AgNOR计数。分析其与喉癌及下咽癌临床分期、病理分级、复发、转移、生存期之间的相关性。结果 :PCNA阳性细胞百分率 (PCNA指数 )与AgNOR计数呈正相关 ;PC NA指数与AgNOR计数由早期到晚期 ,由高分化到低分化逐渐提高 ;两者在复发、转移及期内死亡患者的癌组织中增加 (P <0 .0 5 )。结论 :PCNA指数和AgNOR计数可作为喉及下咽鳞癌的诊断性预测指标。

Expression of PCNA and the count of AgNOR in squamous cell carcinoma of larynx and hypopharynx

OBJECTIVE: 1. To investigate the expression of PCNA (Proliferating Cell Nuclear Antigen) and the count of AgNOR(Nucleolar Organized Region) in respectively from larynx and hypopharynx. 2. To analysis the correlation between them and the clinical stage, the histological grade, the relapse, the lymph node involving and the survival period. 3. To evaluate their value on clinical prognosis. METHOD: Immunohischemical staining was used to detect the expression of PCNA. AgNOR was detected by using Crocker's staining. The lesion tissue and normal tissue specimens were obtained from 61 patients with squamous cell carcinoma and 9 of patients with benign tumor respectively. RESULT: The expression of PCNA is correlate with the count of AgNOR. Both of them were increased in patients with advance clinical stage, low different tumor, tumor relapse, lymph nod involving or short survival period (P < 0.05). CONCLUSION: The positive expression rate of PCNA and the count of AgNOR may be used as prediction of tumor malignancy and prognosis in squamous cell carcinoma of larynx and hypopharynx.