GC法测定醋氯芬酸含量

目的建立气相色谱法测定醋氯芬酸原料的含量的方法。方法气相色谱内标法。色谱柱为DB-1弹性毛细管柱(30 m×0.53 mm,1.5μm),FID检测器;汽化室温度为260℃,检测器温度300℃;柱温采用程序升温,载气为氮气,流速为5.0 mL.min-1,尾吹30 mL.min-1,分流比10∶1.以咖啡因为内标物。结果醋氯芬酸在10～110μg.mL-1(r=0.9998)范围内呈良好线性关系,平均回收率为100.1%。结论本方法简便,快速,准确,可靠,可用于醋氯芬酸的含量测定。

Determination of acedofenac by GC

OBJECTIVE To establish a GC method for determination of aceclofenac elasticity.The internal standard GC method was applied.METHODS The chromatographic system consisted of DB-1 capillary column and FID as detector.The injector temperature was 260℃,the detector temperature was 300℃;the flow rate of N2 was 5.0mL·min-1,make up flow was 30mL·min-1,split ratio was 10∶1.Caffeine was used as internal standard.RESULTS A good linearity of aceclofenac was obtained between 10～110μg·mL-1（r=0.9998）.The average recoveries was 100.1%.CONCLUSION The method was simple,rapid and accurate,and can be used for the determination of aceclofenac.