习惯性自发性流产女性的染色体结构稳定性研究

目的分析习惯性自发性流产女性和正常女性的染色体结构稳定性的不同,为提高生育质量提供理论依据。方法随机选择习惯性自发性流产的女性20人为观察组及正常的育龄女性20人为对照组,对其外周血淋巴细胞按姐妹染色单体互换(SCE)检测方法、微核检测方法及染色体断裂检测方法进行培养检测,分别记数SCE发生率、微核出现率及染色体断裂发生率。结果观察组的外周血淋巴细胞SCE发生率和微核出现率明显高于对照组(P<0.05);观察组的外周血淋巴细胞的染色体断裂发生率与对照组比较差异不明显(P>0.05)。结论SCE发生率和微核出现率可以作为经常暴露在易致DNA损伤的环境下发生习惯性自发性流产女性染色体结构稳定性的检测指标。

Study the stability of chromosome structures in women with recurrent spontaneous abortions

Objective To analyze the difference of stability of chromosomic structure between women with recurrent respontaneous abortions and normal women at childbearing age in order to provide the theory, basis for the enhancement birth quality. Methods Twenty women with recurrent spontaneous abortions and twenty normal women with childbearing age were selected randomly as observation group and control group respectively. The peripheral blood lymphocytes from them were cuhivated and detected by sister ehromatid exchanges（SCE） mieronueleus test method and chromosome breakage test method. And the ineidenee of SCE,the frequency of mieronueleus and the incidence of chromosome breakage were recorded. Results The incidence of SCE and the frequency of micronucleus in observation group were higher than that in control group（ P 〈 0.05） ,but there was no significant difference in the incidenee of chromosome breakage between two groups（P 〉 0.05）. Conclusion The incidence of SCE and the frequency of micronueleus can be used as the detective index of chromosome＇s structural stability of women with recurrent spontaneous abortions who are exposed to the environment which can results in DNA damage.