人参养荣汤对亚急性老化小鼠肝及脑组织线粒体形态结构保护作用的研究

目的研究人参养荣汤对亚急性老化小鼠肝、脑细胞线粒体形态结构变化的保护作用。方法40只ICR系小鼠随机分成青年对照组、模型组、人参养荣汤(中药)高、中、低3个剂量组,观察电子显微镜下的线粒体超微结构变化,并通过医学图像分析仪对线粒体比表面积、体密度、面数密度、比膜面积及嵴数目进行定量的分析。结果电镜观察模型组线粒体数目减少,线粒体肿胀、扩张明显,嵴断裂、畸形、溶解、消失现象明显,符合衰老鼠线粒体形态改变；不同剂量中药组随用药浓度增加,线粒体数目增多,肿胀显著改善,嵴及内膜的衰老变性改变明显减轻。结论人参养荣汤可以通过改善小鼠肝、脑细胞线粒体随增龄所产生的结构变化而起到抗衰老的作用。

Protective Effect of Renshen Yangrong Decoction on Morphology and Structure of Mitochondria in Brain and Liver of Subacute Senile Mice

Objective To study the effect of Renshen Yangrong decoction (RYD) in protecting morphology and structure of mitochondria in brain and liver of D-galactose induced subacute senile mice.Methods Forty ICR mice were randomly divided into the youth control group,the model group,the high,middle and low-dose RYD groups.The ultra-structure changes of mitochondria were observed by electronic microscope,the specific surface area,body density,numerical density on area,δmmit area and ridge count of mitochondria were qu...