| 环孢A处理对脊髓损伤大鼠后肢骨骼肌RAF-1表达的影响 |
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| 引用本文： | 王波,李玉,但齐琴,王廷华.环孢A处理对脊髓损伤大鼠后肢骨骼肌RAF-1表达的影响[J].昆明医学院学报,2011,32(4):6-9. |
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| 作者姓名： | 王波 李玉 但齐琴 王廷华 |
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| 作者单位： | 1. 昆明医学院第一附属医院神经外科,云南,昆明,650031
2. 昆明医学院神经科学研究所,云南,昆明,650031 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的探讨环孢霉素A处理对脊髓损伤大鼠后肢骨骼肌RAF-1 mRNA表达的影响.方法成年SD大鼠分SCT组（T9横断）,和SCT后给予环孢霉素A处理组.脊髓横断组用环孢霉素A处理（1 mg/200 g）7 d后处死动物.取后肢骨骼肌用免疫组化染色（n=5）确定RAF-1 mRNA在后肢骨骼肌的定位分布.RT-PCR技术检测RAF-1 mRNA在损伤脊髓大鼠后肢骨骼肌的表达变化（n=8）.结果与手术组比较（0.95±0.13）,环孢霉素A处理（0.75±0.14）导致RAF-1 mRNA在损伤脊髓大鼠后肢骨骼肌的表达明显下调（P〈0.05）;RAF-1蛋白产物主要分布在细胞浆.结论环孢霉素A处理能有效下调脊髓损伤大鼠后肢骨骼肌RAF-1 mRNA表达水平.
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| 关 键 词： | 环孢霉素A 脊髓全横断 RAF-1mRNA 免疫组化 RT-PCR 大鼠 |
Effects of Cyclosporine A on RAF-1 Expression in Skeletal Muscles of Hindlimbs in Adult Rats after Spinal Cord Transaction |
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| WANG Bo,LI Yu,DAN Qi-qin,WANG Ting-hua.Effects of Cyclosporine A on RAF-1 Expression in Skeletal Muscles of Hindlimbs in Adult Rats after Spinal Cord Transaction[J].Journal of Kunming Medical College,2011,32(4):6-9. |
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| Authors: | WANG Bo LI Yu DAN Qi-qin WANG Ting-hua |
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| Institution: | WANG Bo,LI Yu,DAN Qi-qin,WANG Ting-hua(1)Dept.of Neurosurgery,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650031,2)Institute of Neuroscience,Kunming Medical University,China) |
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| Abstract: | Objective To investigate the effects of cyclosporine A on RAF-1 expression in the skeletal muscles of hindlimbs in adult rats after spinal cord transaction.Methods Sprague-Dawley(SD)rats were randomly divided into operation group(T9 transection)and CSA treatment group.After spinal cord transection(SCT)was performed,CSA(1 mg/200 g)was intrapentoneously injected one time every day to rats in CSA treatment group till 7 days after SCT.The spinal cords were harvested,then RT-PCR(n=8)and immunohistochemistry(n=5)... |
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| Keywords: | RAF-1 mRNA RT-PCR |
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