| 20（R）-人参皂苷Rg_3抗血小板活化因子诱导血管内皮细胞损伤的保护作用 |
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| 引用本文： | 喻卓,陈鹏,杨桂梅,何波,杨莉,沈志强. 20（R）-人参皂苷Rg_3抗血小板活化因子诱导血管内皮细胞损伤的保护作用[J]. 昆明医学院学报, 2011, 32(5): 7-12 |
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| 作者姓名： | 喻卓 陈鹏 杨桂梅 何波 杨莉 沈志强 |
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| 作者单位： | 1. 昆明医学院第一附属医院心内科,云南,昆明,650032
2. 昆明医学院云南省天然药物药理重点实验室,云南,昆明,650031 |
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| 基金项目： | 云南省自然科学基金资助项目 |
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| 摘 要： | 目的探讨20（R）-人参皂苷Rg3抗血小板活化因子（PAF）损伤人脐静脉血管内皮细胞（HUVEC）的保护作用及其机制.方法采用MTT法测定PAF诱导HUVEC活性;用双波长荧光分光光度法测定HUVEC细胞内游离钙离子浓度;用ELISA法测定培养的细胞上清液中t-PA、PAI-1含量.结果 1μmol/LPAF刺激HUVEC 2 h后,可明显降低HUVEC的吸光度值,细胞内钙离子浓度升高,上清液中PAI-1含量明显升高,而t-PA含量无明显变化;20～80μmo/L 20（R）-人参皂苷Rg3可升高HUVEC吸光度值,并呈浓度依赖性显著降低HUVEC内游离钙浓度;20（R）-人参皂苷Rg3降低上清液中PAI-1含量,对t-PA含量无明显影响.结论 20（R）-人参皂苷Rg3对PAF诱导损伤的HUVEC具有抗损伤保护作用,其机理可能与降低细胞内钙离子浓度,抑制PAI-1产生和调节t-PA/PAI-1平衡作用有关.
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| 关 键 词： | 20（R）-人参皂苷Rg3 血小板活化因子 血管内皮细胞 t-PA/PAI-1 |
Protective Effects of 20(R)-ginsenoside Rg3 against PAF-induced Injury of Human Umbilical Vein Endothelial Cells |
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| YU Zhuo,CHEN Peng,YANG Gui-mei,HE Bo,YANG Li,SHEN Zhi-qiang. Protective Effects of 20(R)-ginsenoside Rg3 against PAF-induced Injury of Human Umbilical Vein Endothelial Cells[J]. Journal of Kunming Medical College, 2011, 32(5): 7-12 |
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| Authors: | YU Zhuo CHEN Peng YANG Gui-mei HE Bo YANG Li SHEN Zhi-qiang |
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| Affiliation: | YU Zhuo1),CHEN Peng2),YANG Gui-mei2),HE Bo2),YANG Li2),SHEN Zhi-qiang2)(1)Dept.of Cardiology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,2)Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming Yunnan 650031,China) |
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| Abstract: | Objective To investigate the protective effect of 20(R)-ginsenoside Rg3 against PAF-induced human umbilical vein endothelial cell(HUVEC)injury and its preliminary mechanism.Methods Platelet activating factor(PAF)was used to injure HUVEC in vitro.Cell viability was assessed with methyl thiazolyl tetrazolium(MTT)assay.The cultured cells were loaded by Fura-2/AM and the change of i in HUVEC was measured by fluorospectrophotometry.The levels of tissue plasminogen activator(t-PA)and plasminogen activator inhibit... |
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| Keywords: | t-PA/PAI-1 |
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