| TRB3在同型半胱氨酸抑制内皮细胞增殖中的作用研究 |
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| 引用本文： | 单妍,田兴亚,张伟,梁璇,刘佳,邰文琳.TRB3在同型半胱氨酸抑制内皮细胞增殖中的作用研究[J].昆明医学院学报,2011,32(4):16-19. |
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| 作者姓名： | 单妍 田兴亚 张伟 梁璇 刘佳 邰文琳 |
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| 作者单位： | 1. 昆明医学院海源学院,云南,昆明,650101
2. 昆明医学院第二附属医院检验科,云南,昆明600101 |
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| 基金项目： | 云南省教育厅及昆明医学院海源学院基金资助项目 |
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| 摘 要： | 目的研究TRB3对介导同型半胱氨酸的血管内皮细胞抑制的影响.方法用3H]-胸腺嘧啶脱氧核苷掺入法测定细胞DNA合成率,基因表达测定采用RT-PCR,蛋白质磷酸化采用Western blotting.结果同型半胱氨酸处理人脐静脉内皮细胞导致细胞增殖被抑制;同型半胱氨酸诱导的内质网应激,导致CHOP和ATF4表达升高;同型半胱氨酸上调TRB3表达,导致Akt的去磷酸化.结论 TRB3是同型半胱氨酸介导的血管内皮细胞的细胞周期停滞的关键分子.
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| 关 键 词： | 同型半胱氨酸 内皮细胞 细胞增殖 TRB3 Akt |
TRB3 Mediates Homocysteine-induced Inhibition of Endothelial Cells Proliferation |
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| SHAN Yan,TIAN Xing-ya,ZHANG Wei,LIANG Xuan,LIU Jia,TAI Wen-lin.TRB3 Mediates Homocysteine-induced Inhibition of Endothelial Cells Proliferation[J].Journal of Kunming Medical College,2011,32(4):16-19. |
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| Authors: | SHAN Yan TIAN Xing-ya ZHANG Wei LIANG Xuan LIU Jia TAI Wen-lin |
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| Institution: | SHAN Yan 1),TIAN Xing-ya1),ZHANG Wei 1),LIANG Xuan1),LIU Jia1),TAI Wen-lin2)(1)Dept.of Biochemistry,Haiyuan College of Kunming Medical University,Kunming Yunnan 650106,2) Dept.of Clinic Chemistry,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China) |
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| Abstract: | Objective To investigate the effect of TRB3 on homocysteine-induced inhibition of endothelial cells proliferation.Methods DNA synthesis in cells was determined by using -thymidine incorporation.The expression of tested genes was detected by real-time PCR.The phosphorylation of proteins was measured by Westren blotting.Results(1)Homocysteine inhibited the proliferation of human umbilical vein endothelial cells(HUVECs).(2)Homocysteine induced endoplasmic reticulum stress,and up-regulated the expression of CHO... |
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| Keywords: | TRB3 Akt |
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