缓激肽B2受体基因多态性对膝关节骨关节炎易感性的影响

背景：据报道在膝骨关节炎患者中应用缓激肽B2受体拮抗剂能产生持久的镇痛效果，缓激肽B2受体(BDKRB2)在骨关节炎的发展中具有重要作用。 目的：进一步明确缓激肽B2受体基因多态性与骨关节炎易感性的相关性。 方法：共有278例膝关节骨关节炎患者和291名健康志愿者参与试验。测定受试对象的缓激肽B2受体基因多态性，根据基因型和放射学分型进行统计学处理。 结果与结论：骨关节炎组和对照组中+9/-9 bp多态性的基因型分布和等位基因频率差异明显。-9/-9 bp 多态性和+9/+9 bp相比，与骨关节炎的罹患风险和严重程度存在明显相关性。-9 bp 等位基因的表达，与骨关节炎的Kellgren-Lawrence分型相关。结果提示缓激肽B2受体基因+9/-9 bp多态性可能成为骨关节炎易感性和严重性筛查的分子标记。  中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Bradykinin B2 receptor gene polymorphisms influence the susceptibility of knee osteoarthritis

BACKGROUND: It has been reported that bradykinin B2 receptor (BDKRB2) antagonists can produce long-lasting analgesic effect in patients with knee osteoarthritis, and BDKRB2 plays an important role in the development of osteoarthritis. OBJECTIVE: To further verify the association BDKRB2 polymorphisms and osteoarthritis susceptibility. METHODS: 278 patients with primary knee osteoarthritis and 291 healthy volunteers as controls were enrolled. BDKRB2 gene polymorphisms were genotyped. RESULTS: The genotype distributions and allele frequencies of +9/-9 bp polymorphisms significantly differed between osteoarthritis patients and healthy controls. The -9/-9 polymorphisms were significantly associated with the increased risk and the radiographic severity of knee osteoarthritis compared to the +9/+9 genotype. The presence of -9 bp was associated with higher Kellgren-Lawrence grade in knee osteoarthritis. These findings suggest that the +9/-9 bp polymorphisms of BDKRB2 gene may be used as a molecular marker for the susceptibility and severity of knee osteoarthritis.