叶酸与维生素B12对2型糖尿病大鼠心肌组织凋亡 相关蛋白表达的影响

摘要:目的　观察2型糖尿病大鼠心肌凋亡相关蛋白Bax、Bcl-2和Caspase-3的表达变化及叶酸、维生素B12的干预作用。方法　6周龄SD大鼠60只，糖尿病造模成功后，随机分为对照组（A组）、模型组（B组）、叶酸和维生素B12干预组（C组）。12周后取血检测各组同型半胱氨酸（Hcy）、叶酸、维生素B12的水平；取大鼠心肌组织，免疫组化法检测Bax，Bcl-2，Caspase-3的表达，并用计算机图像分析系统测平均灰密度值。结果　12周后B组Hcy明显增高，与A组和C组比较，差异均有统计学意义（P＜0.01），C组叶酸、维生素B12与A组和B组比较，差异均有统计学意义（P＜0.01）；心肌组织中，B组Bax、Caspase-3的表达明显高于A组（P＜0.01），Bcl-2的表达明显低于A组（P＜0.01）；C组Bax、Caspase-3的表达较B组明显下降（P＜0.01），Bcl-2的表达明显高于B组（P＜0.01）；A组与C组Bax、Caspase-3、Bcl-2的表达差异无统计学意义（P＞0.05）。结论　2型糖尿病大鼠血液中Hcy的升高，可能在促进心肌细胞凋亡过程中发挥重要作用，而叶酸、维生素B12的干预可以有效缓解病变进程。

The influence of folic acid and vitamin B12 on the expression of apoptosis-related proteins in myocardial tissues of type II diabetic rats

Abstract: Objective The study aimed to observe the intervening effects of folic acid and vitamin B12 on the changes in expression of apoptosis-related proteins, including Bax, Bcl-2, and caspase-3, in myocardial tissues of type II diabetic rats. Methods 60 diabetic model rats were successfully created using SD rats over 6 weeks of age and were randomly divided into a control group (Group A), a model group (Group B), and an intervention group with folic acid and vitamin B12 (Group C). The levels of blood homocysteine (Hcy), folic acid, and vitamin B12 in each of the groups were measured after 12 weeks of intervention. The expressions of Bax, Bcl-2, and caspase-3 were determined by immunohistochemistry, and the average gray density were measured by using a computer image analysis system. Results After 12 weeks of intervention, Hcy in Group B significantly increased as compared with Group A and Group C (P<0.01). Folic acid and vitamin B12 levels in Group C were significantly different than those of Group A and Group B (P<0.01). In the myocardial tissue, Group B had significantly higher expressions of Bax and caspase-3 than Group A (P<0.01), and significantly lower expression of Bcl-2 than Group A (P<0.01). Group C had lower expressions of Bax and caspase-3 (P<0.01) and higher expression of Bcl-2 (P<0.01) than Group B. No significant differences in expressions of Bax, caspase-3, and Bcl-2 were observed between Group A and Group C (P>0.05). Conclusion Elevated level of Hcy in blood of type II diabetic rats may play an important role in promoting apoptosis of myocardial cells. Intervention with folic acid and vitamin B12 can effectively delay the progression of the disease.