卵胞浆内单精子显微注射受精失败的原因及对策

背景：卵胞浆内单精子显微注射(ICSI)已经成为治疗男性因素不育的首选方法，且具有很高的成功率，但卵胞浆内单精子显微注射周期的受精失败发生率仍有1%-3%。目的：简要综述有关卵胞浆内单精子显微注射受精失败的相关因素及其对策。方法：检索万方数据库、中国知网及PubMed1994年1月至2015年6月与卵胞浆内单精子注射相关研究的文献。阅读文题和摘要，依据纳入排除标准对其中的60篇进行分析探讨。结果与结论：目前，研究发现卵母细胞激活失败与卵子异常、精子缺陷、患者年龄、显微操作技术及实验室环境等因素密切相关。辅助卵母细胞激活是解决卵胞浆内单精子显微注射后激活失败的关键技术。了解卵胞浆内单精子显微注射受精失败的相关因素后对其采取相应的对策，可以提高卵胞浆内单精子显微注射受精率及改善胚胎发育潜能。 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 

Fertilization failure following intracytoplasmic sperm injection: causes and countermeasures

BACKGROUND: Intracytoplasmic sperm injection (ICSI) has become the first choice for treatment of male infertility. Despite its high success rates, fertilization failure following ICSI still occurs in 1%-3% of couples.OBJECTIVE: To review the relevant factors and countermeasures of fertilization failure in ICSI.METHODS: Wanfang, CNKI and PubMed databases were searched for articles related to ICSI published from January 1994 to June 2015. Finally, 60 articles meeting the inclusive criteria were included in result analysis. RESULTS AND CONCLUSION: Oocyte activation failure is closely related to abnormal eggs, sperm defects, age, micromanipulation technology and laboratory environment. Assisted oocyte activation is the gordian technique to solve the fertilization failure in ICSI. To understand the reasons for fertilization failure in ICSI and to take appropriate measures can promote the rate of fertilization and improve the potential of embryonic development.