Periostin对自体脂肪移植成活率的影响

目的 探索Periostin复合自体脂肪颗粒移植后,对移植脂肪血管化及成活率的影响,以明确Periostin在自体脂肪移植中的作用。方法 体外实验验证低氧条件下Periostin对脂肪干细胞成血管的影响,RT-PCR检测CD133及CD144的基因表达情况;将Periostin复合自体脂肪组织移植到裸鼠体内,观察移植脂肪的成活情况。结果在低氧条件下,Periostin组细胞成血管能力明显优于空白对照组;RT-PCR检测结果显示,Periostin组的CD133和CD144表达量明显高于空白对照组;Periostin复合自体脂肪移植后1、3、6个月,移植脂肪成活率分别为79.50%±3.70%、67.71%±4.08%和64.78%±5.78%,明显高于对照组(51.83%±5.97%、39.45%±10.32%和34.20%±10.61%)。结论 Periostin能够改善脂肪移植早期组织对缺氧的耐受性,促进自体脂肪颗粒移植后的早期血管化,有利于移植脂肪颗粒的存活,可有效改善脂肪移植的成活率。

Effect of Periostin on the Survival Rate of Autologous Fat Transplantation

Objective To explore the effect of Periostin on the vascularization and survival rate of autologous fat transplantation, and to find out the role of Periostin in autologous fat transplantation. Methods The special effect of Periostin in inducing vascularization of adipose stem cells under hypoxia was observed in vitro. The expression of CD133 and CD144 of ASCs under hypoxia were tested by RT-PCR. Then, the adipose tissue compounded with Periostin was transplanted into nude mice, and the survival rate of the transplanted fat tissue was detected at different time points after transplantation.Results Periostin group showed a higher vascularization degree under hypoxia, compared with blank control group. The expression of CD133 and CD144 of ASCs under hypoxia in Periostin group were higher than in blank control group. Periostin composite with autologous fat transplantation improved the survival rate of fat transplantation at each time point（79.50% ±3.70%, 67.71% ±4.08%, 64.78% ±5.78%）, which were better than simple autologous fat transplantation（51.83% ±5.97%,39.45% ±10.32%, 34.20% ±10.61%）. Conclusion Periostin is able to improve fat survivability under hypoxia during the early stage, and promote the early stage vascularization of autologous fat granules after transplantation, which is beneficial to the survival of the transplanted fat tissue.