七氟醚预处理对缺血再灌注损伤大鼠的 神经保护作用及机制研究

摘要:目的　通过观察七氟醚预处理对大鼠TNF-α和IL-1β蛋白含量的影响，从而探讨七氟醚预处理对缺血再灌注损伤大鼠的神经保护作用及机制。方法　将36只健康雄性SD大鼠随机分为假手术组、损伤组及七氟醚组。对损伤组及七氟醚组采用右颈内动脉尼龙线线栓法行大脑中动脉阻闭模型（MCAO），七氟醚组在制备MCAO模型前的24 h吸入七氟醚（浓度为2.5%七氟醚及97.5%氧气）60 min。假手术组只分离右侧颈总动脉及颈外动脉结扎，不进入颅内置入线栓。大鼠缺血2 h后，进行再灌注24 h。比较3组大鼠的神经功能缺陷评分及脑组织TNF-α和IL-1β蛋白含量。结果　假手术组所有大鼠神经功能缺陷评分均为0分；损伤组41.7%（5/12）的大鼠评分为2分、50%（6/12）大鼠评分为3分，8.3%（1/12）的大鼠评分为4分；七氟醚组大鼠33.3%（4/12）评分为4分，41.7%（5/12）评分为2分，25.0%（3/12）评分为3分。3组大鼠的评分分布差异具有统计学意义（χ2＝15.637，P＜0.001）。3组大鼠脑组织TNF-α总体水平差异具有统计学意义（F＝10.375，P＜0.01），IL-1β蛋白含量总体水平差异具有统计学意义（F＝23.174，P＜0.01），不论TNF-α和IL-1β蛋白含量，两两比较后各组间差异均具有统计学意义，损伤组均高于七氟醚组及假手术组。结论　七氟醚预处理对局灶性脑缺血再灌注损伤具有延迟性保护作用，其主要机制与降低TNF-α和IL-1β等炎症因子蛋白含量有关。

Research on sevoflurane pretreatment on ischemia-reperfusion injury in rats nerve protective effect and mechanism

Abstract: Objective To observe the Sevoflurane preconditioning effects in rats TNF alpha and beta IL-1 protein levels, and to discuss the protective effect and mechanism of sevoflurane pretreatment on ischemia-reperfusion injury rats nerve. Methods 36 healthy male SD rats were randomly divided into control, damage and sevoflurane group. The jury and sevoflurane group were disposed by using right internal carotid artery nylon lines plug line closed model (MCAO). Sevoflurane group inhaled sevoflurane (concentration of 2.5% sevoflurane and 2.5% oxygen) 60 min in the preparation of the 24 h before the MCAO model. The control group only separated of the right common carotid artery and external carotid artery ligation without the intracranial placement line bolt. 24h reperfusion after 2h ischemia. The rats nerve function score and brain tissue TNF alpha and beta IL-1 protein levels of the three groups were compared. Results All control group rats nerve function score was 0. The rate of 2 score, 3 score and 4 score of the injury group was 41.7%, 50% and 8.3%, respectively. The rate of 2 score, 3 score and 4 score of the sevoflurane group was 41.7%, 25.0% and 3.3%, respectively. The score of the three groups was significantly different (χ2=15.637, P<15.637). The level of TNF alpha of the three groups was significantly different (F=10.375, P<0.01). IL-1 beta protein levels was significantly different (F=23.174, P<0.01). There was significant difference between any two of the three group. sevoflurane group and injury group were higher than control group. Conclusion Sevoflurane pretreatment has delayed protection for focal cerebral ischemia-reperfusion injury. The main mechanism is to reduce TNF alpha and beta IL - 1 inflammatory factor related protein levels.