纳米/微米SiO2颗粒致肺腺癌A549细胞炎性反应因子的变化

摘要:目的　探讨纳米/微米SiO2颗粒单独及复合暴露对A549细胞的炎性反应的影响。方法　采用体外细胞培养的方式，建立人肺腺癌细胞A549与纳米/微米SiO2单独、复合暴露模型。以染毒浓度为6.25、25.0及100 μg/ml分别为实验的低、中、高剂量组，一次性染毒24 h后，通过ELISA法检测细胞上清液中炎性反应因子IL-6、IL-8及TNF-α水平的变化。结果　各低、中、高剂量下，纳米/微米单独或复合暴露均可引起A549细胞上清液中炎性反应因子IL-6、IL-8及TNF-α水平升高，且存在剂量-反应关系（P＜0.05）。3个指标总体均呈现单独暴露组之间纳米组细胞上清液中炎性反应水平高于同剂量微米组（P＜0.05）；两种粒径纳米SiO2颗粒相比，同浓度30 nm组对细胞炎性损伤作用较50 nm组高（P＜0.05）；复合暴露组细胞炎性反应水平高于同剂量单独暴露组（P＜0.05）的趋势。结论　纳米/微米SiO2颗粒单独或复合暴露均可引起人肺腺癌细胞A549内炎性反应因子的释放，产生炎性损伤。

Nano/micro SiO2 Particles induced human pulmonary adenocarcinoma A549 cell inflammatory factors change

Abstract: Objective To investigate the nano/micro SiO2 particles separate and composite exposure on the inflammatory response of A549 cells. Methods By way of in vitro cell culture method, the establishment of human lung adenocarcinoma cell line A549 and nano/micro SiO2 single, composite exposure models. With exposures concentration 6.25, 25.0 and 100μg/ml were set for the experimental low, medium and high dose groups. After 24h exposure one-time, cell supernatants were detected inflammatory factor IL-6, IL-8 and TNF-α levels by ELISA. Results Nano/micro exposed singly or in combination could cause an inflammatory reaction in the cell supernatant A549 factor IL-6, IL-8 and increased TNF-α levels in a dose- response relationship (P<0.05). Three indicators showed overall between individual cells exposed to inflammatory reaction supernatant. Nano group was higher than the same dose micron group (P<0.05). Compared to the size of nano-SiO2 particles in two, the inflammatory injury of concentration of 30nm group was higher than that of 50nm (P<0.05). The inflammatory response of complex cells exposed group was higher compared with a single dose exposure group (P<0.05). Conclusion Nano/micro SiO2 particles alone or in combination can cause the release of inflammatory factors in the A549 human lung adenocarcinoma cells, and then produce inflammatory injury.