体外磁共振观察胎鼠神经干细胞的标记

背景：神经干细胞移植后的细胞存活、识别和迁移需动态监控。 目的：通过体外磁共振技术对胎鼠神经干细胞体外标记，为神经干细胞在神经系统修复中的应用提供依据。 方法：采用胎鼠神经干细胞的分离与培养标记、染色剂鉴定及神经干细胞的活性检测，构建大鼠脑缺血再灌注模型，采用超顺磁性氧化铁颗粒体外标记胎鼠的神经干细胞并移植至模型大鼠左侧脑内，未标记的胎鼠神经干细胞移植至右侧脑中，对标记的细胞进行普鲁士蓝染色，观察其定植和迁移情况，并通过磁共振示踪动态的监测神经干细胞在活体移植之后的信号改变情况。 结果与结论：超顺磁性氧化铁颗粒体外标记胎鼠神经干细胞的方法效率达95%以上，电镜结果显示超顺磁性氧化铁颗粒体外标记胎鼠的神经干细胞内含铁颗粒，且集中在溶酶体和内涵体当中，磁共振结果显示胎鼠标记的神经干细胞呈现低信号改变，细胞活性的影响与未标记组差异无显著性意义，但标记的胎鼠神经干细胞T2WI 与 T2*WI信号降低。证实超顺磁性氧化铁颗粒体外标记胎鼠的神经干细胞可高效表达，磁共振的监控可以用于神经干细胞的活体示踪。   中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

In vitro labeled neural stem cells of fetal rats: MRI observation

BACKGROUND:It is necessary to dynamically monitor the survival, recognition and migration of neural stem cells after implantation. OBJECTIVE:To in vitro label fetal rat neural stem cells using MRI technology so as to provide applied evidence of neural stem cells in nervous system repair. METHODS:Fetal rat neural stem cells were isolated, cultured and labeled followed by identification and cell viability detection. A rat model of cerebral ischemia-reperfusion injury was established. Fetal neural stem cells labeled by superparamagnetic iron oxide particles in vitro were transplanted into the left brain of model rats, and unlabeled fetal rat neural stem cells transplanted into the right brain. Prussian blue staining was used to observe the colonization and migration of implanted neural stem cells. MRI tracing was employed to monitor the signal changes of neural stem cells dynamically after in vivo transplantation. RESULTS AND CONCLUSION:Over 95% fetal rat neural stem cells were labeled successfully by superparamagnetic iron oxide particles, and under electron microscope, there were iron particles in labeled neural stem cells, which were concentrated in the lysosome and endosome. MRI results showed that the labeled neural stem cells had a changing trend of low signals. No difference was found in the cell viability between labeled and unlabeled cells, but T2WI and T2*WI signals were reduced in labeled neural stem cells. These findings confirm that superparamagnetic iron oxide-labeled fetal rat neural stem cells can highly express, and MRI tracing can be used for in vivo monitoring of neural stem cells.