儿童炎症性肠病病原菌分析

摘要:目的　分析某院炎症性肠病（Inflammatory Bbowel Disease，IBD）儿童患者粪便中的菌群分布，并与正常对照进行比较，探讨儿童IBD的发生与肠道菌群的关系。方法　选取2011年5月-2014年8月在该院确诊的IBD患儿共101例作为调查对象；同时选取该院同一时间段进行健康体检的健康儿童50例作为对照。收集调查样本新鲜粪便，通过荧光定量PCR检测肠道优势菌群的数量情况，比较溃疡性结肠炎（UC）患儿、克罗恩病（CD）患儿与正常对照组各优势菌种的数量差异。结果　根据菌群定量检测结果发现，拟杆菌属在3组人群中数量差异无统计学意义（F＝3.148，P＞0.05），UC组及CD组双歧杆菌、缠绕杆菌、乳酸菌、脆弱杆菌、梭状芽胞杆菌比对照组显著减少（F值分别为15.331、17.453、21.479、18.521，27.658，P值均＜0.05），UC组及CD组肠球菌比正常对照组明显增多（F＝23.457，P＜0.05）。结论　儿童肠道优势菌群与IBD的发生具有关联性，但优势菌群分布与IBD发生的因果关系还需要今后进行进一步的研究证实。

Analysis of inflammatory bowel disease pathogen in children

Abstract: Objective This work was to analyze the bacterial flora in the feces of inflammatory bowel disease (IBD) children patientsby comparing it with the normal control, so as to explore the correlation between children IBD and the gut microflora. Methods A total of 101 IBD patients were selected from Xiaguanying Central Public Health Center during may, 2011 ~ August 2014. At the same time, 50 healthy children taken physical examination served as as the control subjects. Fresh feces samples were collected from the subjects for fluorescence quantitative PCR detection of the predominant bacteria. Comparison was made between the results of children with ulcerative colitis (UC) or crohn's disease (CD) and that of the control group. Results Quantitative test results found that three groups had no significant difference in the amount of bacteroides in the gut microflora (F=3.148, P>0.05). However, in comparison to the control group, the UC and CD groups had much less level of Bifidobacteria, Lactobacillus, Bacillus fragilis, and Clostridium (F=15.331、21.479、18.521 and 27.658, respectively. P<0.05), but had much more Enterococci (F=23.457, P<0.05). Conclusion Children gut microflora is correlated with IBD, but the mechanism of such correlation is to be revealed.