神经干细胞缺氧/复氧损伤中的低氧诱导因子1α和诱导型一氧化氮合酶

背景：在缺氧状态可诱导机体产生缺氧诱导因子，而诱导型一氧化氮合酶表达产生的一氧化氮(NO)可抑制低氧诱导因子1α的活性。 目的：探索低氧诱导因子1α、诱导型一氧化氮合酶与神经干细胞的低氧/复氧损伤关系。 方法：无菌条件下将出生24 h以内的Wistar大鼠处死，并分离大鼠海马组织，制作成脑组织的细胞悬液，分离培养神经干细胞后，将细胞分为常氧组、低氧组及低氧-复氧组，后两组以150 μmol/L氯化钴溶液制备低氧模型，而后低氧-复氧组细胞在低氧4 h时复氧。 结果与结论：低氧条件下，神经干细胞凋亡数量及细胞中低氧诱导因子1α和诱导型一氧化氮合酶 mRNA表达水平增加；而缺氧-复氧组中凋亡神经干细胞数量高于缺氧组，但低氧诱导因子1α和诱导型一氧化氮合酶mRNA表达水平低于缺氧组。说明这两种因子参与细胞低氧损伤过程。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：

Role of hypoxia-inducible factor 1 alpha and inducible nitric oxide synthase in neural stem cells during hypoxia/reoxygenation injury

BACKGROUND: Organism in the hypoxic state can produce hypoxia-inducible factor, but nitric oxide generated from inducible nitric oxide synthase can inhibit the activity of hypoxia-inducible factor 1α. OBJECTIVE: To discuss the relationship of hypoxia-inducible factor 1α and inducible nitric oxide synthase with hypoxia/reoxygenation injury in neural stem cells. METHODS: Under sterile conditions, Wistar rats born within 24 hours were sacrificed to separate the rat hippocampus that was used to prepare a cell suspension of brain tissue. After culture and passage, neural stem cells were divided into normoxia, hypoxia and hypoxia/reoxygenation groups. In the latter two groups, 150 μmol/L cobalt chloride solution was used to prepare hypoxia models, and in the hypoxia/reoxygenation group, the cells were reoxygenated after 4-hour hypoxia. RESULTS AND CONCLUSION:Under hypoxic conditions, a significant increase in mRNA expressions of hypoxia-inducible factor 1α and inducible nitric oxide synthase as well as the number of apoptotic neural stem cells. Compared with the hypoxia group, the number of apoptotic neural stem cells was higher in the hypoxia/reoxygenation group, but the mRNA expressions of hypoxia-inducible factor 1α and inducible nitric oxide synthase were lower. These findings indicate that these two factors are involved in the hypoxia/reoxygenation injury of neural stem cells.