| 志贺菌中成簇的规律间隔短回文重复序列的相关基因检测 |
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| 引用本文： | 薛泽润1,2,王颖芳3,段广才2,4,杨海燕2,郗园林2,王鹏飞2,王琳琳2,郭向娇2. 志贺菌中成簇的规律间隔短回文重复序列的相关基因检测[J]. 现代预防医学, 2015, 0(18): 3371-3372 |
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| 作者姓名： | 薛泽润1 2 王颖芳3 段广才2 4 杨海燕2 郗园林2 王鹏飞2 王琳琳2 郭向娇2 |
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| 作者单位： | 1.西安市疾病预防控制中心病毒科，陕西 西安 710054；2.郑州大学公共卫生学院，河南 郑州 450001；3.河南科技大学医学院预防医学系，河南 洛阳 471023；4.新乡医学院分子诊断与医学检验技术河南省协同创新中心，河南 新乡 453003 |
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| 摘 要： | 摘要:目的 建立检测志贺菌CRISPR相关基因的斑点杂交方法。方法 分别采用PCR 法制备地高辛标记DNA 探针和人工合成的寡核苷酸探针，与志贺菌CRISPR相关基因进行斑点杂交。结果 设计出志贺菌CRISPR的相关基因探针；实现了志贺菌CRISPR系统的探针制备及杂交方法的建立。结论 斑点杂交可以快速大量检测志贺菌CRISPR的相关基因。
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| 关 键 词： | 关键词:志贺菌 CRISPR 斑点杂交 寡核苷酸探针 |
Dot blot hybridization for the detection of CRISPR in Shigella |
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| XUE Ze-run,WANG Ying-fang,DUAN Guang-cai,YANG Hai-yan,XI Yuan-lin,WANG Peng-fei,WANG Lin-lin,GUO Xiang-jiao. Dot blot hybridization for the detection of CRISPR in Shigella[J]. Modern Preventive Medicine, 2015, 0(18): 3371-3372 |
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| Authors: | XUE Ze-run WANG Ying-fang DUAN Guang-cai YANG Hai-yan XI Yuan-lin WANG Peng-fei WANG Lin-lin GUO Xiang-jiao |
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| Affiliation: | *Xi'an Center for Disease Control and Prevention, Xi'an, Shanxi 710054, China |
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| Abstract: | Abstract: Objective This work was to establish a dot-blot hybridization for the detection of clustered regularly interspaced short palindromic repeat (CRISPR) with DNA probe in Shigella. Methods PCR was carried out to prepare the digoxingenin (DIG) -labeled DNA probe and oligonucleotide probe to detect CRISPR associated genes in Shigella by dot blot hybridization. Results Probes for CRISPR associated genes in Shigella were designed successfully. The establishment of probe and hybridization of Shigella CRISPR system was completed. Conclusion Dot blot hybridization is a rapid and sensitive method for the detection of CRISPR in Shigella. |
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| Keywords: | Keywords: Shigella CRISPR system Dot blot hybridization Oligonucleotide probe |
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