基因芯片技术在乙型肝炎病毒和拉米夫定耐药性检验中的应用

目的：评价基因芯片技术在乙型肝炎(乙肝)基因分型和拉米夫定耐药性检测中的临床价值。方法：选择287例乙肝病毒(HBV)免疫学标志物阳性患，用PCR法筛选HBV-DNA阳性血清，用肝炎基因诊断芯片进行基因分型，并对拉米夫定治疗中YMDD基因变异进行分析。结果：153例HBV-DNA阳性中，B基因型8例，C基因型144例，B／C混合型1例；拉米夫定治疗1年以上、3个月～1年、少于3个月的YMDD突变率分别为41．46％、16．00％和3．20％。结论：肝炎基因芯片可用于乙肝诊断分型，并可对村米夫定治疗后耐药性讲行判断。

Application of Gene Chip to Detection of Hepatitis B Virus and Lamivudine Drug Resistance Test

Objective:To evaluate the clinical role of DNA microarray in detection of HBV genotpes and Lamivudine drug resistance test. Methods:HBV-DNA positive surum was selected by PCR from 287 cases with positive immunological markers for HBV. Then DNA microarray of hepatitis B virus was applied to detect HBV genotypes and to analyze YMDD genovariation in Lamivdine treatment. Results:Of all 153 cases with positive HBV-DNA,8 B genotypes, 144 C genotypes and 1 B/C genotypes were found respectively; YMDD genocariation rate on different course of Lamivudine treatment, over one year, three months to one year and shorter than three months, were 41.66% , 16.00% and 3.20% ,respectively. Conclusions:Hepatitis DNA microarray was applicable to diagnosis of hepatitis B virus genotypes and to judgment of drug resistance after Lamivudine treatment.