| 甲醇法制备的血模板DNA用于聚合酶链反应 |
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| 引用本文： | 赵彤,朱慧萍,李勇. 甲醇法制备的血模板DNA用于聚合酶链反应[J]. 卫生研究, 2001, 30(2): 115-116 |
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| 作者姓名： | 赵彤 朱慧萍 李勇 |
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| 作者单位： | 北京大学生育健康研究所，北京 100083 |
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| 基金项目： | 国家自然科学基金(No.39800130) |
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| 摘 要： | 建立从外周血标本制备用于聚合酶链反应的 DNA模板的甲醇法 ,并与 A/ B液法、Qiagen纯化柱法和 ISOCODE法进行比较。甲醇制备纸血片 DNA法为 :取直径为 3mm的血环 ,经甲醇固定后加去离子水 ,煮沸后离心 ,上清即为 DNA储备液。甲醇法与其它 3种制备模板 DNA的方法比较 ,具有提取的模板 DNA质量较高 ,血标本容易获得、保存且成本低 ,制备效率高 ,而且实验方法简单易行等诸多优点。
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| 关 键 词： | 甲醇 模板DNA 聚合酶链反应 外周血 |
| 文章编号： | 1000-8020(2001)02-0115-02 |
| 修稿时间： | 2000-06-28 |
Methanol method for the preparation of template DNA from blood for polymerase chain reaction |
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| Zhao Tong,Zhu Huiping,Li Yong Institute of Reproductive Health,Beijing University,Beijing ,China. Methanol method for the preparation of template DNA from blood for polymerase chain reaction[J]. Journal of hygiene research, 2001, 30(2): 115-116 |
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| Authors: | Zhao Tong Zhu Huiping Li Yong Institute of Reproductive Health Beijing University Beijing China |
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| Affiliation: | Institute of Reproductive Health, Beijing University, Beijing 100083, China. |
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| Abstract: | A methanol method for the preparation of template DNA for polymerase chain reaction(PCR)from peripheral blood specimens was established A diameter of 3 mm blood spots on paper were punched and fixed in methanol for overnight Deionized water was then added After boiling and centrifuging, the supernatants reserved DNA. The blood blot of specimen is easy to be obtained and reserved in low cost. The experiment technique is high in efficiency . |
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| Keywords: | methanol template DNA polymerase chain reaction peripheral blood |
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