| 膜片钳技术和酶法分离豚鼠初级精母细胞的比较 |
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| 引用本文： | 李云阁,李云义,沙建慧,刘焕珠,沈楠,战术,姜丽萍.膜片钳技术和酶法分离豚鼠初级精母细胞的比较[J].吉林医药学院学报,1999(1). |
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| 作者姓名： | 李云阁 李云义 沙建慧 刘焕珠 沈楠 战术 姜丽萍 |
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| 作者单位： | 空军医学高等专科学校!吉林市132013(李云阁，李云义，沙建慧，刘焕珠，沈楠，姜丽萍)，白求恩医科大学!长春市130021(战术) |
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| 摘 要： | 应用膜片钳技术与酶法分离细胞技术,分别获得豚鼠单个初级精母细胞,观察了光镜下不同浓度伴刀豆蛋白A、不同时间对精母细胞贴壁及经膜表面清洁后封接和破膜的影响.结果表明,膜片钳技术优于酶法分离,细胞贴壁采用0.125g/L伴刀豆蛋白A作用10min为宜,膜表面清洁处理以0.35g/L胶原酶作用5min易于封接和破膜.
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| 关 键 词： | 精母细胞 分离 膜片钳技术 |
Comparison of patch-clamp technique with enzymatic dissociation in isolating primary spermatocytes in guinea pigs |
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| Li Yunge,Li Yunyi,Sa Jianhui,et al Air Force Medical College,Jilin City.Comparison of patch-clamp technique with enzymatic dissociation in isolating primary spermatocytes in guinea pigs[J].Journal of Jilin Medical College,1999(1). |
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| Authors: | Li Yunge Li Yunyi Sa Jianhui Air Force Medical College Jilin City |
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| Institution: | Li Yunge,Li Yunyi,Sa Jianhui,et al Air Force Medical College,Jilin City,132013 |
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| Abstract: | Single primary spermatocyles in guinea pigs were obtained both by patch-clamp technique and by enzymatic dissociation and observed under microscope for the effects of concanavalin A in different concentrations and for the influence of different time lengths on its setdement to the bottom of 35 mm Petri dishes.The result showed that patch-clamp technique is superior to enzymatic dissociation, the suitable concentration was 0.125 g/L and the suitable time length was 10 min; for cleaning the surface of membrane,0.35g/L pipan was suitable and 5 min was enough. |
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| Keywords: | spermatocyte dissociation patch-clamp technique |
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