Searching for a New Putative Cryptic Virus in Pinus sylvestris L |
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Authors: | Dorina Veliceasa Natalya Enünlü Péter B Kós Sigrid Köster Eckhardt Beuther Bogdan Morgun Sachin D Deshmukh Noémi Lukács |
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Institution: | (1) Biological Research Center, Institute of Plant Biology, H-6701 Szeged, Hungary;(2) Institute of Physical Biology, Heinrich Heine University, 40225 Düsseldorf, FRG;(3) Department of Plant Physiology and Plant Biochemistry, Corvinus University Budapest, Villányi út 29-43, H-1118 Budapest, Hungary |
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Abstract: | Double-stranded RNAs (dsRNAs) were detected in different pine populations in Germany and Hungary. Two dsRNA species of 1.5
and 1.58 kbp, respectively, persisted in the same trees for at least 2 years and their presence was not associated with any
symptoms. The dsRNAs were found to sediment in the VLP (virus-like particles) fraction and to be protected by protein(s) against
RNase A digestion at low salt. cDNA cloning and sequencing of the smaller segment (dsRNA2) led to the identification of a
putative RNA-dependent RNA-polymerase (RdRp) containing the GDD, as well as three other, conserved motifs. Sequence comparison
with different RNA viruses and phylogenetic analysis indicates that the putative RdRp from pine shows highest similarity to
the homologous proteins of Beet cryptic virus 3 and of a cryptic virus of Pyrus pyrifolia. On the basis of these results we suggest that the 1.5 and 1.58 kbp dsRNAs in P. sylvestris may represent the genomic segments of a new plant cryptic virus, Cryptoviruses have not yet been reported to occur in Gymnosperms. |
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Keywords: | Cryptovirus dsRNA Pinus sylvestris Partitiviridae RNA-dependent RNA polymerase |
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