祛风通络方对阿霉素肾病大鼠肾小球足细胞nephrin mRNA表达的影响

目的：观察阿霉素肾病(AN)大鼠肾小球足细胞nephrin mRNA表达及祛风通络方对其表达的影响。方法：清洁级SD大鼠140只，随机抽取32只作为空白组，余108只建立AN大鼠模型，90只大鼠模型成功后随机分为模型组、祛风组、祛风+激素组、激素组和苯那组，给予相应干预，电镜检测超微结构变化，第0，3，5，7周应用间接免疫荧光技术、RT-PCR分别检测nephrin蛋白荧光表达分布和nephrin mRNA表达并进行组间比较。结果：模型成功时，透射电镜示AN大鼠肾小球足突呈部分或弥漫性融合。7周时与模型组相比，祛风组和激素组nephrin蛋白的荧光分布呈连续性或斑片状分布，荧光强度明显增强(P＜0.01)；祛风组和激素组nephrin mRNA的表达明显上调(P＜0.01)。结论：Nephrin的表达异常是AN大鼠蛋白尿发生发展的重要分子机制，祛风通络方可上调AN大鼠足细胞nephrin的表达和分布而起保护肾小球滤过屏障的作用。

Effect of Qufengtongluo recipe on expression of nephrin mRNAin adriamycin-induced nephropathy in rats

Objective To investigate the expression of nephrin mRNA in adriamycin-induced nephropathy (AN) in rats, and to explore the effect of Qufengtongluo recipe on proteinuria in AN rats and on the expression of nephrin mRNA. Methods Adriamycin nephropathy was induced by a single tail intravenous injection of adriamycin. We randomly divided 140 rats into a normal control group (n=32) and a nephropathy model group (n=108). Three weeks later, 90 AN rats were randomly divided into 5 groups: a model group, a qufeng group, a qufeng and prednisone group, a prednisone group, and a benazepri group (18 rats in each group). They were treated respectively, and renal tissue samples were collected at week 0, 3, 5, and 7, respectively. The distribution and expression of nephrin mRNA were examined by indirect immunofluorescence and semi-quantity RT-PCR. Results In the AN rats, the diffuse fusion and effacement of foot processes were observed at week 3. The fluorescence intensity of nephrin and the expression of nephrin mRNA significantly increased in the qufeng group and the prednisone group compared with the model group at the week 7 (P＜0.01).Conclusion Abnormal expression of nephrin is the important molecular mechanism that leads to the occurrence and development of proteinuria in AN rats. Qufengtongluo recipe has effect on nephrotic syndrome through altering the expression and distribution of nephrin in glomerulus.