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从丹参中筛选血管紧张素转换酶抑制剂
引用本文:高小平,徐大勇,邓义龙,张燕. 从丹参中筛选血管紧张素转换酶抑制剂[J]. 中国中药杂志, 2004, 29(4): 359-362
作者姓名:高小平  徐大勇  邓义龙  张燕
作者单位:成都地奥制药集团,药物研究所,四川,成都,610041
摘    要:目的 :从丹参中筛选血管紧张素转换酶抑制剂 (ACEI)。方法 :分别提取丹参水溶性和脂溶性部分 ,用荧光检测法筛选抑制血管紧张素转换酶 (ACE)活性的有效部位或成分。结果 :丹参水溶性部分、丹参总酚酸及丹酚酸B显著降低了大鼠肺组织的ACE活性 ,其IC50 分别为 ( 2.45± 0.07) ,( 0.24± 0.02 ) ,( 0.02± 0.01)g·L-1。含丹参酮Ⅰ ,Ⅱ的菲醌类成分或脂溶性部分没有作用。 结论 :丹参具有ACEI作用 ,其活性成分存在水溶性部分中 ,主要为含丹酚酸B等酚酸类物质。

关 键 词:丹参  丹参总酚酸  丹酚酸B  血管紧张素转换酶抑制剂
文章编号:1001-5302(2004)04-0359-04
收稿时间:2003-09-26

Screening of angiotensin converting enzyme inhibitors from Salvia miltiorrhizae
GAO Xiao ping;XU Da yong;DENG Yi long;ZHANG Yan. Screening of angiotensin converting enzyme inhibitors from Salvia miltiorrhizae[J]. China Journal of Chinese Materia Medica, 2004, 29(4): 359-362
Authors:GAO Xiao ping  XU Da yong  DENG Yi long  ZHANG Yan
Affiliation:Institute of Materia Medica, Chengdu DIAO Group, Chengdu 610041, China. gxplww@hotmail.com
Abstract:OBJECTIVE: To screen angiotensin converting enzyme inhibitor (ACEI) from traditional Chinese medicine, Salvia miltiorrhiza. METHOD: Hydrophilic and lipophilic fractions of S. miltiorrhiza were isolated, and their effective components were screened by a fluorimetric assay for inhibition of angiotensin converting enzyme (ACE). RESULT: Water-soluble fractions, total salvianolic acids and salvianolic acid B markedly decreased ACE activity of rabbit lung tissue. Their IC50 value were (2.45 +/- 0.07), (0.24 +/- 0.02), (0.02 +/- 0.01) g x L(-1) respectively. Lipophilic components or phenanthraquinones including tanshinone I and II showed no changes on the activity of ACE. CONCLUSION: S. miltiorrhiza inhibits angiotensin converting enzyme and its active components are in aqueous extract, in which the main were salvianolic acids including salvianolic acids B.
Keywords:Salvia miltiorrhiza  total salvianolic acids  salvianolic acid B  angiotensin converting enzyme inhibitor
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