高效液相色谱法测定咳愈口服液巾愈创木酚甘油醚、右美沙芬等四组分的含量

目的：采用高效液相色谱法测定咳愈口服液中马来酸氯苯那敏、盐酸甲基麻黄碱、愈创木酚甘油醚、氢溴酸右美沙芬的含量。方法：色谱柱：Agilent C8柱，流动相为甲醇-磷酸二氢钾溶液（20mmol／L．pH3.0）=35：65．检测波长220nm。结果：马来酸氯苯那敏在1.275-12．75μg／ml，盐酸甲基麻黄碱在14．0～140μg／ml，愈创木酚甘油醚在63.0-630μg／ml，氢溴酸右美沙芬在18.75～187．5μg／ml范围内，均有良好的线性关系。平均回收率分别为100.6％．99.2％，99．4％．98.9％。结论：本法简便，准确．重现性好，可用于咳愈口服液的质量控制。

HPLC Determination of Chlorphenamine,Methylephedrine,Guaifenesin and Dextromethorphan in Keyu Oral Solution

Objective： An HPLC method for determination of chlorphenamine maleate, methylephedrine hydrochloride, guaifenesin and dextromethorphan hydrobromide in Keyu oral solution was established. Methods： The chromatographic conditions were as follows： Agilent Cs column, methanol-20mmol/L phosphate buffer solution （pH 3.0） （35：65） as the mobile phase and UV detector at 220 nm. Results： The calibration curve were linear in the range of 1.275-12.75μg/ml for chlorphenarnine maleate, 14.0-140μg/ml for methylephedrine hydrochloride, 63.0-630μg/ml for guaifenesin and 18.75-187.5μg/ml for dextromethorphan hydrobromide. The average recoveries were 100.6%, 99.2%, 99.4%, 98.9% respectively. Conclusion： The method is simple, accurate and reliable for the quality control of Keyu oral solution.