IL-10基因多态性与冠状动脉疾病相关性研究

目的 探讨白细胞介素-10(IL-10)基因启动子区-1082位点多态性与冠状动脉疾病(CAD)相关性及其对血清IL-10水平的影响.方法 采用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)检测50例CAD患者、60例健康对照者基因组DNA IL-10基因启动子区域-1082位点的基因多态性,并进行相关性分析.用双抗体夹心ELISA法测定血清IL-10水平.结果 CAD组血清IL-10水平显著低于健康对照组(P＜0.05).CAD组和健康对照组IL-10-1082A/G基因型和等位基因频率比较,差异有显著性意义.等位基因频率的相对风险分析显示,A等位基因携带者患CAD的相对风险是G等位基因的2.827倍.携带A等位基因的CAD患者血清IL-10水平显著低于非携带者.结论 IL-10基因启动子-1082位点多态性影响血清IL-10水平并可能与CAD的临床发展过程相关,其中A等位基因可能是CAD发病的遗传易感基因.

Relationship between Polymorphism of IL-10-1082 Gene and Coronary Atherosclerotic Heart Diseases

Objective To investigate the correlation between polymorphism of IL-10-1082 gene and coronary artery diseases(CAD) in Chinese population.Methods Using PCR-RFLP assay,a 1082A/G single nucleotide polymorphism(SNP) of the anti-inflammatory IL-10 gene was analyzed.The polymerase chain reaction(PCR) technique was used to study the polymorphisms of IL-10 gene and allele frequencies in 50 patients with CAD and 60 age-matched Chinese healthy controls.Results In CAD group,serum level of IL-10 was significantly lower than in aged-control group.The allele A and genotype AA frequencies of-1082 locus in IL-10 promoter region were significantly higher in CAD patients than those in healthy aged-controls.Relative risk analysis of IL-10 allele revealed that the relative risk of allele A carriers suffering CAD was 2.827 times of that of allele A carries.Conclusion The genetic polymorphism of IL-10-1082 may be associated with CAD.The IL-10 allele A carriers may be at the increased risk of CAD due to the suppression of the IL-10 expression.