基于遗传算法的土香薷总黄酮提取工艺优化研究 |
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引用本文: | 朱文瑞,吴巧凤,宓嘉琪,韩柱. 基于遗传算法的土香薷总黄酮提取工艺优化研究[J]. 浙江中医药大学学报, 2014, 0(10): 1207-1211 |
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作者姓名: | 朱文瑞 吴巧凤 宓嘉琪 韩柱 |
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作者单位: | 浙江中医药大学药学院 杭州 310053 |
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基金项目: | 国家自然科学基金资助项目(81274030/H2803,81202926/H2806);浙江省自然科学基金项目(LY12H27008);浙江省大学生科技创新活动计划暨新苗人才计划资助项目(2013R410019) Fund project:The National Natural Science Foundation of China(81274030/H2803,81202926/H2806); Zhejiang Provincial Natural Science Foundation,Scheme of scientifitic and technological innovation activities and Xinmiao Talents for Zhejiang Province college students |
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摘 要: | [目的]确定土香薷总黄酮的最优提取工艺。[方法]以乙醇浓度、料液比、提取时间和提取次数为考察因素,以总黄酮含量为目标函数,运用SPSS18.0软件建立其正交试验数据的二次回归模型,并进一步采用遗传算法对其进行优化,确定土香薷总黄酮的最优工艺条件。[结果]建立总黄酮含量(Y)与各因素的二次回归模型为:Y=51.039+2.164X3X4-0.007X1X2;遗传算法优化后得其最优提取工艺条件为:加药材21倍量浓度为61%的乙醇提取3次,每次2h。;此条件下土香薷总黄酮的平均含量为54.37 mg/g,RSD为1.29%,与预测值(53.71mg/g)基本相近。[结论]所选最优提取条件提取率高、稳定性好,可用于土香薷总黄酮的提取。
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关 键 词: | 遗传算法 正交试验 土香薷 总黄酮 工艺优化 |
Optimization of the Extraction Process in Total Flavonoids from Origanum Vulgare Linn. By the Genetic Algorithm |
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Affiliation: | Zhu Wenrui, Wu Qiaofeng, Mi Jiaqi, et al( Pheutical College, Zhejiang Chinese Medical University, Hangzhou310053) |
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Abstract: | Objective] To optimize the extraction process in Origanum vulgare Linn. [Methods] With ethanol concentration, solid-liquid ratio, extraction time and extraction times to examine factors, using the content of total flavonoids as the objective function, the orthogonal experiment was optimized the extraction process. SPSS18.0 software was used to establish its quadratic regression model from orthogonal test data. Using genetic algorithm to search the optimum process conditions and the optimization of extraction technology of the total flavones was carried out by GA. [Results]Its quadratic regression model was Y=51.039+2.164X3X4-0.007X1X2. The optimum extraction conditions were confirmed as fol owing: 61 times volume ethanol, 21% of the ethanol, to extract 3 times and 2h for each time. Under these conditions, the yield of total flavonoids was 54.37mg/g, little difference in predictive value (53.71mg/g). [Conclusion] The advantages of this process are efficiency, stability and reliability, which can be applied in the optimization of extraction process of total flavonoids in Origanum vulgare Linn. |
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Keywords: | genetic algorithm orthogonal design Origanum vulgare Linn total flavonoids process optimization |
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