| HPLC法测定苦参浸膏中苦参碱和氧化苦参碱的含量 |
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| 引用本文： | 周玉枝,原红霞. HPLC法测定苦参浸膏中苦参碱和氧化苦参碱的含量[J]. 世界中西医结合杂志, 2010, 5(4): 315-316,342 |
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| 作者姓名： | 周玉枝 原红霞 |
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| 作者单位： | 1. 山西中医学院第二中医院,山西,太原,030024
2. 山西中医学院,山西,太原,030024 |
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| 摘 要： | 目的考察苦参不同时间水煎后苦参碱和氧化苦参碱的含量变化,为苦参在复方中的应用提供参考。方法采用HypersiL NH2(250 mm×4.6 mm,5μm)柱,流动相为乙腈-无水乙醇-3%磷酸(85∶7.5∶7.5),流速为1.0 mL.min-1,检测波长为210 nm,进样量为10μL。结果苦参随着煎煮时间的延长,苦参碱的含量增加,氧化苦参碱的含量变化不大。结论在苦参提取过程中,水煎时间不是影响氧化苦参碱转化为苦参碱的主要因素。
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| 关 键 词： | 苦参 苦参碱 氧化苦参碱 高效液相色谱法 |
Determination of Contents of Matrine and Oxymatrine in Flavescens Extract by HPLC |
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| ZHOU Yu-zhi,YUAN Hong-xia. Determination of Contents of Matrine and Oxymatrine in Flavescens Extract by HPLC[J]. World Journal Of Integrated Traditional and Wesrern Medicine, 2010, 5(4): 315-316,342 |
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| Authors: | ZHOU Yu-zhi YUAN Hong-xia |
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| Affiliation: | ZHOU Yu-zhi1,YUAN Hong-xia2 (1.No.2 Chinese Medicine Hospital of Shanxi College of Traditional Chinese Medicine,Taiyuan Shanxi 030024,2.Shanxi College of Traditional Chinese Medicine,Taiyuan Shanxi 030024) |
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| Abstract: | Objective To observe the changes in the contents of matrine and oxymatrine after decocting flavescens for different times so as to provide the reference for the application of flavescens in compound formula.Methods HypersiL NH2（250 mm × 4.6 mm,5 μm）column was adopted.Ethanol-3% phosphoric acid（85∶7.5∶7.5）was taken as a mobile phase,the flow rate was 1.0 mL·min-1,and the detecting wavelength was 210 nm.Sample volume was 10 μL.Results While flavescens was decocted for longer time,matrine content increased and oxymatrine content did not change greatly.Conclusion In flavescens extraction,the time of decoction is not the chief factor to impact the transformation of matrine from oxymatrine. |
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| Keywords: | Flavescens Matrine Oxymatrine HPLC |
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