c-erbB-2、bcl-2蛋白在宫颈鳞癌细胞中的表达及其意义

目的　研究c erbB 2、bcl 2基因在宫颈鳞癌中的表达及其在宫颈癌发生、发展中的作用及意义。方法　采用免疫组织化学S P法分别检测 4 5例宫颈鳞癌组织、10例宫颈上皮内瘤变和 10例正常宫颈鳞状上皮中c erbB 2、bcl 2蛋白的表达。结果　c erbB 2、bcl 2蛋白在宫颈鳞癌中的阳性表达率分别为 5 5 .5 6 %、2 8.2 9% ;在CIN中的阳性表达率分别为 0、10 .0 0 % ;在NE组均无表达。c erbB 2蛋白在宫颈鳞癌中阳性表达与在CIN、NE中的表达差异有显著性 (P <0 .0 1) ;bcl 2蛋白在宫颈鳞癌中阳性表达与在CIN中的表达及在NE中的不表达差异均无显著性 ( P >0 .0 5 ) ;c erbB 2与bcl 2之间在宫颈鳞癌组织中的阳性表达呈负相关 ( P <0 .0 5 )。结论　c erbB 2、bcl 2基因参与了宫颈鳞癌的发生、发展过程。c erbB 2基因蛋白的过表达是宫颈组织恶性变的重要生物学标志。联合检测c erbB 2、bcl 2基因蛋白对判断宫颈癌预后有一定意义

The expression and clinical significances of c-erbB-2,bcl-2 proteins in squamous cell carcinoma of the uterine cervix

Objective To study the expression of c erbB 2,bcl 2 proteins in squamous cell carcinoma of the uterine cervix and their clininical significances. Methods The expression of c erbB 2,bcl 2 proteins was detected by S P immunohistochemical methods in 45 cases of squamous cell carcinoma of the uterine cervix and 10 cases of cervical intraepithelial neoplasia(CIN) and 10 samples of normal cervical squamous epithelium(NE). Results The positive expression rates of c erbB 2,bcl 2 were 58.33%,26.67% respectively in squamous cell carcinoma of the uterine cervix,and were 0, 10.00% in CIN,and both were absent in NE.The expression of c erbB 2 had significant difference between squamous cell carcinoma of the uterine cervix and CIN or NE(P<0.01),but the expression of bcl 2 had not difference(P>0.05). There was negative relationship between expression of c erbB 2 and bcl 2 in cervical carcinoma(P<0.05). Conclusions c erbB 2,bcl 2 proteins may play an important role in the development and progression of squamous cell carcinoma of the uterine cervix.The overexpression of c erbB 2 proteins might be a biological marker for malignant transformation in cervical tissue. .The combined detection of c erbB 2,bcl 2 geneproteins has positive effects on predicting the prognosis of cervical carcinoma.