辛基酚对MCF-7乳腺癌细胞周期及周期蛋白表达的影响

目的观察辛基酚对MCF-7乳腺癌细胞周期及周期蛋白表达的影响。方法以MTT试验、流式细胞分析、免疫细胞化学和RT-PCR等方法观察辛基酚对MCF-7乳腺癌细胞增殖、细胞周期相和细胞凋亡、CDK2和CDK4 mRNA表达、Cyclin D1 mRNA及蛋白表达的影响。结果4、8、16μmol/LOP作用MCF-7乳腺癌细胞72h时,细胞增殖率分别为107.31%、168.06%、62.00%,G0/G1期细胞分别为(52.46±6.67)%、(50.19±7.39)%、(67.31±5.47)%,对照组(55.27±7.53)%,细胞凋亡率分别为(4.84±1.12)%、(6.48±1.36)%、(19.26±3.57)%,对照组(5.56±1.125)%,16μmol/LOP减少了细胞中CDK2、CDK4、Cyclin D1 mRNA及Cyclin D1的表达。结论4、8μmol/LOP促进MCF-7乳腺癌细胞增殖,16μmol/LOP则抑制细胞增殖,促进细胞凋亡,这可能与OP影响细胞中CDK2、CDK4、Cyc-lin D1 mRNA及Cyclin D1表达有关。

Effect of Octylphenol on Cell Cycle and Cyclin Expression on MCF-7 Breast Cancer Cells

Objective To observe the effect of octylphenol(OP) on cell proliferation, cell cycle and cyclin expression of MCF-7 breast cancer cells.Methods Using MTT test, flow cytometry, immunocytochemistry and RT-PCR to observe the changes of cell proliferation, cell cycle, apoptosis, CDK2,CDK4 and Cyclin D1 mRNA expression, Cyclin D1 protein expression of MCF-7 cells.Results When MCF-7 breast cancer cells were treated with 4,8 and 16μmol/L OP on 72h, the proliferation ratio of MCF-7 was 107.31%、168.06% and 62.00% respectively , MCF-7 cell at G₀ / G₁ were (52. 46 ±6. 67) %、(50. 19 ±7. 39) % and (67. 31 ±5. 47) % respectively ,while cont rol group was (55. 27 ±7. 53) %. The apoptosisratio of MCF-7 was (4. 84 ±1. 12) % , (6. 48 ±1. 36) % and (19. 26 ±3. 57) % respectively ,and the control group was (5. 56 ±1. 125) %. The expression of CDK2 ,CDK4 and Cyclin D1 mRNA significant decreased when MCF27 breast cancer cells were t reated with 16μmol/ L OP , the expression of Cyclin D1protein decreased too. Conclusion 　The proliferation of MCF-7 breast cancer cells increases when t reatedwith 4 ,8μmol/ L OP , but it decreases when t reated with 16μmol/ L OP. These different effect s on MCF-7 cells maybe related to the expressive regulation of CDK2 , CDK4 and Cyclin D1.