Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies. |
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Authors: | A G Coulepis M F Veale A MacGregor M Kornitschuk I D Gust |
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Abstract: | Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which human polyclonal serum was used. The monoclonal antibody assay proved to be more sensitive for the detection of hepatitis A virus from fecal specimens as well as for anti-hepatitis A virus immunoglobulin G (IgG) and IgM in sera. |
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