Detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays |
| |
Authors: | Jin Lian-Qun Li Jun-Wen Wang Sheng-Qi Chao Fu-Huan Wang Xin-Wei Yuan Zheng-Quan |
| |
Affiliation: | 1. PLA Center of Disease Control and Prevention, Beijing 100039, China 2. Tianjin Institute of Hygiene and Environmental Medicine, Tianjin 300050, China 3. Beijing Institute of Radiation Medicine, Beijing 100850, China |
| |
Abstract: | AIM: To detect the common intestinal pathogenic bacteria quickly and accurately. METHODS: A rapid (<3 h) experimental procedure was set up based upon the gene chip technology. Target genes were amplified and hybridized by oligonucleotide microarrays. RESULTS: One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 (96.2%) were identified. CONCLUSION: Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, Proteus sp., Bacillus cereus, Vibrio choterae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonucleotides to the arrays without any significant increase of complexity or cost. |
| |
Keywords: | Oligonucleotide array Sequence analysis Gene chip Intestines Microbiology |
本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录! |
|