HMB-45抗原在毛囊黑素细胞中的表达

用单克隆抗体ＨＭＢ－４５，以ＡＰＡＡＰ法对２０份头皮活检标本的９８个毛囊组织进行染色。其中２０个毛囊为生长早期，６３个为成熟生长期，７个为退行期和８个是休止期。结果，５２个成熟生长期及２０个生长早期毛囊的黑素细胞与单克隆抗体ＨＭＢ－４５显著结合。所有退行期、休止期及工１１个成熟生长期毛囊未被染色。结果提示；ＨＭＢ－４５在毛囊中的表达随毛发生过周期而变化，且与黑素细胞功能活性状态有关。

Expression of HMB-45 Antigen in melanocytes of Hair Follicles

In the present study, biopsy specimens of the scalp of 20 patients were stained with antibody directed against HMB--45. 20 out of 98 hair bulbs were in the early anagen phase, 63 were mature anagen, 7 were catagen and 8 showed a typical telogen feature. In 52 out of 63 mature anagen and all 20 early anagen hair bulbs, the melanocytes were strongly stained with HMB--45. In contrast, melanocytes in 11 mature anagen bulbs as well as in catagen or telogen phase were not stained. Our findings suggest that the expression of HMB--45 antigen in melanocytes of hair bulbs is strongly related to the cell cycle of hair,i. e. HMB--45 antigen only binds to the active or proliferating melanocytes in early and mature anagen hair follicles,but not to the inactive or dedifferentiated melanocytes in catagen of telogen hair follicles.