大豆异黄酮改善高血脂小鼠记忆的研究

目的:探讨大豆异黄酮(SI)对高血脂小鼠记忆的影响及有关机制。方法:将小鼠分为4组,基础饲料对照组;高脂饲料对照组;低剂量SI组:高脂饲料+SI(50mg/kgbw);高剂量SI组:高脂饲料+SI(100mg/kgbw)。连续灌胃SI30d后,测定小鼠记忆能力,并检测血清总胆固醇(TC)、甘油三酯(TG)和β-脂蛋白含量及超氧化物歧化酶(SOD)活力,血液、大脑皮质和海马中胆碱酯酶(AChE)活性以及大脑皮质和海马中氨基酸神经递质(Asp、Glu和Gly)含量。结果:在实验期内造成高脂模型,与对照组比较,高血脂小鼠记忆明显降低,而SI明显改善高血脂小鼠记忆能力,同时使小鼠血清中TC、TG和β-脂蛋白含量以及大脑皮质和海马中AChE活力显著降低,而血清SOD活力及大脑皮质和海马中氨基酸神经递质含量显著提高。结论:SI可改善高血脂小鼠的记忆,其机制可能与其抗氧化作用及调节神经递质的代谢有关。

STUDY ON SOYBEAN ISOFLAVONES IMPROVING MEMORY OF HYPERCHOLESTEROLEMIA MICE

Objective: To study the effect of soybean isoflavones ( SI ) on memory of hypercholesterolemia mice and the involved mechanism. Method: 64 mice were randomly divided into four groups: control group, high lipid group, low dosage SI (50mg/kg bw) group for high lipid animal, and high dosage SI (100mg/kg bw) group for high lipid animal. The experimental period was 30d. Memory function of experimental mice, and its serum total cholesterol(TC), triglyceride(TG), β-lipoprotein level, SOD and AChE activity, and AChE activity and amino acid neurotransmitter (Asp, Glu and Gly) level of cerebral cortex and hippocampus were determined. Result: The hypercholesterolemia mice had poor memory function, and SI improved it, meanwhile, blood TC, TG, β-lipoprotein, AChE activity, and AChE activity of cerebral cortex and hippocampus were decreased by SI treatment. Whereas, serum SOD activity and Asp, Glu and Gly contents of cerebral cortex and hippocampus were increased significantly. Conclusion: SI could improve memory function of hypercholesterolemic mice, and the mechanism involved might closely correlate with antioxidation and modulating the metabolism of neurotransmitters in central nerve system.