| 三尖杉内生真菌次生代谢产物的活性成分分析 |
| |
| 引用本文： | 陈文强,刘兆迪,程贤利,解修超,邓百万,彭浩,乔艳明.三尖杉内生真菌次生代谢产物的活性成分分析[J].中国实验方剂学杂志,2015,21(12):54-57. |
| |
| 作者姓名： | 陈文强 刘兆迪 程贤利 解修超 邓百万 彭浩 乔艳明 |
| |
| 作者单位： | 陕西理工学院 生物科学与工程学院, 陕西 汉中 723001;陕西省食药用菌工程技术研究中心, 陕西 汉中 723001,陕西理工学院 生物科学与工程学院, 陕西 汉中 723001,汉中职业技术学院 药学与医学技术系, 陕西 汉中 723000,陕西理工学院 生物科学与工程学院, 陕西 汉中 723001;陕西省食药用菌工程技术研究中心, 陕西 汉中 723001,陕西理工学院 生物科学与工程学院, 陕西 汉中 723001;陕西省食药用菌工程技术研究中心, 陕西 汉中 723001,陕西理工学院 生物科学与工程学院, 陕西 汉中 723001;陕西省食药用菌工程技术研究中心, 陕西 汉中 723001,陕西理工学院 生物科学与工程学院, 陕西 汉中 723001 |
| |
| 基金项目： | 陕西省教育厅专项科研计划项目(14JK1150) |
| |
| 摘 要： | 目的:研究三尖杉内生真菌次生代谢产物的活性成分。方法:采用滤纸片扩散法和噻唑蓝比色法对三尖杉内生真菌CEP18发酵液的乙酸乙酯提取物进行抑菌和细胞毒活性测试,利用GC-MS分析活性成分。结果:三尖杉内生真菌次生代谢产物对枯草芽孢杆菌、金黄色葡萄球菌及人早幼粒急性白血病细胞HL-60的生长抑制作用明显。乙酸乙酯提取物中组分Fr2活性最强,主要成分为乳酸丁酯(2.96%),2-氯杀鼠灵酮(8.65%),(Z)-11-二十烯酰胺(13.90%),1-甲基芘(3.51%)及丁二酸二甲酯(6.34%)、对羟基苯乙酸(8.27%)、反油酸甲酯(10.07%)、亚油酸(5.60%)、棕榈酸(7.54%)。结论:对羟基苯乙酸在三尖杉属植物的相关研究中属首次报道,为微生物药物的研发和三尖杉的资源利用提供参考。
|
| 关 键 词： | 三尖杉 内生真菌 次生代谢产物 对羟基苯乙酸 |
| 收稿时间： | 2014/9/28 0:00:00 |
Active Constitunents Analysis of Secondary Metabolites in Endophytic Fungus from Cephalotaxus fortunei Root |
| |
| CHEN Wen-qiang,LIU Zhao-di,CHENG Xian-li,XIE Xiu-chao,DENG Bai-wan,PENG Hao and QIAO Yan-ming.Active Constitunents Analysis of Secondary Metabolites in Endophytic Fungus from Cephalotaxus fortunei Root[J].China Journal of Experimental Traditional Medical Formulae,2015,21(12):54-57. |
| |
| Authors: | CHEN Wen-qiang LIU Zhao-di CHENG Xian-li XIE Xiu-chao DENG Bai-wan PENG Hao and QIAO Yan-ming |
| |
| Institution: | School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China;Shaanxi Engineering Research Center of Edible and Medicated Fungi, Hanzhong 723001, China,School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China,Department of Pharmaceutical and Medical Technology, Hanzhong Vocational and Technical College, Hanzhong 723000, China,School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China;Shaanxi Engineering Research Center of Edible and Medicated Fungi, Hanzhong 723001, China,School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China;Shaanxi Engineering Research Center of Edible and Medicated Fungi, Hanzhong 723001, China,School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China;Shaanxi Engineering Research Center of Edible and Medicated Fungi, Hanzhong 723001, China and School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China |
| |
| Abstract: | Objective: To study active constitunents of secondary metabolites in endophytic fungus from Cephalotaxus fortunei root. Method: Antibacterial and antitumor activity of ethyl acetate extracts from fermentation liquor of an endophytic fungus (CEP18) in C. fortunei root were tested by filter paper diffusion method and MTT,active ingredients were analyzed by GC-MS. Result: Secondary metabolites in endophytic fungus from C. fortunei root significantly inhibited growth of Bacillus subtilis,Staphylococcus aureus and Human promyelocytic leukemia cells (HL-60).After silica gel column chromatography analysis and activity test,five components (Fr1-Fr5) were isolated from ethyl acetate extracts,the Fr2 was identified as active component,which was mainly composed of butyl lactate (2.96%),2-chloro ketone warfarin (8.65%),(Z)-11-eicosenamide (13.90%),1-methyl pyrene (3.51%),dimethyl succinate (6.34%),4-hydroxyphenylacetic acid (8.27%),elaidic acid-methyl ester (10.07%),linoleic acid (5.60%) and hexadecanoic acid (7.54%). Conclusion: 4-Hydroxyphenylacetic acid is reported for the first time in C. genus at present research and is worthy of further development and utilization. |
| |
| Keywords: | Cephalotaxus fortunei endophytic fungus secondary metabolites 4-hydroxyphenylacetic acid |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
|
