安神补脑液去除维生素B1前后对小鼠镇静、催眠作用及脑内RNA,DNA,蛋白质合成影响的比较

目的:探讨维生素B1(VB1)对安神补脑液功效的影响。方法:实验小鼠按体重随机分为7组,分别为正常组(蒸馏水),安神补脑液高、低剂量组(0.1,0.05 g·kg-1),不含VB1的安神补脑液高、低剂量组(0.1,0.05 g·kg-1),VB1口服液高、低剂量组(0.1,0.05 g·kg-1),每组10只。每日按体重ig给药1次,连续ig 10 d。通过小鼠自主活动实验观察各样品的镇静作用;戊巴比妥钠所致小鼠睡眠实验观察各样品的催眠作用;地衣酚(Orcinol)法测定脑组织中RNA含量、二苯胺显色法测定脑组织中DNA含量、福林酚(Lowry)法测定脑组织中蛋白质的含量。结果:与正常组比较,安神补脑液高、低剂量组、不含VB1的安神补脑液高、低剂量组自主活动次数明显减少,且具有显著性差异(P0.05),而VB1口服液高、低剂量组自主活动次数与正常组相比无显著性差异;安神补脑液高剂量组可明显增加小鼠戊巴比妥钠阈上剂量睡眠时间(P0.05),不含VB1的安神补脑液组对小鼠戊巴比妥钠阈上剂量睡眠时间有增加趋势,其他各组无明显差异;除安神补脑液低剂量组外,各组脑内DNA含量均明显增加(P0.01);不含VB1的安神补脑液高剂量组、VB1口服液高、低剂量组蛋白质含量明显增加(P0.01),其余各组无显著性差异;同时,各组对脑内RNA含量无明显影响。结论:不含VB1的安神补脑液和安神补脑液的镇静、催眠作用效果相当;同时,实验证明了不同工艺制备样品对小鼠脑组织中DNA,蛋白质合成有促进作用,对RNA的含量无影响。

Comparison of Sedative Hypnotic Effects and Influence on RNA, DNA, Protein Synthesis in Mice Brain Tissues of Anshen Bunao Syrup with or Without Vitamin B1

Objective: To explore the influence of vitamin B₁ (VB₁) on the effect of Anshen Bunao syrup. Method: The experimental mice were randomly divided into 7 groups: the control group (distilled water), the high and low-dose Anshen Bunao syrup groups (10, 5 mL·kg^-1), the high and low-dose Anshen Bunao syrup without containing VB₁ groups (10, 5 mL·kg^-1), the high and low-dose VB₁ oral liquid groups (10, 5 mL·kg^-1) of 10 mice each. The corresponding medicines were intragastrically administered to the mice according to body weight once daily for 10 days. The sedative effects of different groups were observed by the locomotor activity in mice; the hypnotic effects were observed by the sleeping time in mice with pentobarbital sodium; the RNA content of brain tissue was measured by orcinol method; the DNA content of brain tissue was determined through diphenylamine colorimetric method, and protein content of brain tissue was determined by lowry method. Result: Compared with the control group, the times of spontaneous motion (the indication of sedation) in mice significantly decreased in the high- and low-dose groups of Anshen Bunao syrup and Anshen Bunao syrup without containing VB₁ (P<0.05). There were no significant differences for the times of spontaneous motion between VB₁ oral liquid groups and the control group. The sleep time by pentobarbital sodium significantly increased in the high-dose Anshen Bunao syrup group (P<0.05). There was an increasing tendency for the sleep time by pentobarbital sodium in Anshen Bunao syrup without containing VB₁ groups. There were no significant differences among other groups. The DNA content of brain increased significantly in all groups except the low-dose Anshen Bunao syrup group. The protein content increased significantly in the high-dose Anshen Bunao syrup without containing VB₁ and high-, low-dose VB₁ oral liquid groups (P<0.01). The RNA content in the brain had no significant difference between any two groups. Conclusion: The sedative-hypnotic effect of Anshen Bunao syrup without containing VB₁ is similar to that of Anshen Bunao syrup. Simultaneously, our results prove that the different processes samples could promote DNA and protein synthesis of brain tissues, but they have no influence on RNA content in mice.