前脑啡肽原信使RNA在自发性高血压大鼠脑内的区域定位(英文)

目的:检测前脑啡肽原信使RNA(PPE mRNA)在16周自发性高血压大鼠(SHR)和Wistar-Kyoto大鼠(WKY)中的不同表达。方法:用地高辛标记的RNA探针做非放射性原位杂交。结果:与WKY相比,SHR的PPE mRNA量在下丘脑,中央灰质腹外侧,孤束核,延髓腹外侧网状核,胸髓等处升高;在中央灰质背侧降低;在黑质致密带,齿状回,海马无变化。结论:PPE mRNA在核团中的变化可能与高血压的发生有关。

Mapping of preproenkephalin mRNA in brain of spontaneously hypertensive rats

AIM: To detect different expression of pre-proenkephalin mRNA (PPE mRNA) in 16-wk-old spontaneously hypertensive rat (SHR) and age-matched normotensive Wistar-Kyoto rat ( WKY) . METHODS: Nonradioactive in situ hybridization was performed using digoxigenin-labeled RNA probe. RESULTS: Compared with WKY rats, PPE mRNA levels of 16-wk-old SHR increased in hypothalamic nuclei ( > 20), amygdaloid nuclei ( > 23), ventrolateral central gray (21.2), reticular substantia nigra (21.5), interpeduncular nuclei ( > 21), nucleus of the solitary tract (30.7), rostro-ventrolateral reticular nucleus ( 29.1), gigantocellu-lar reticular nucleus (23.9) and thoracic spinal cord ( > 30 ) ; decreased in dorsal central gray (22.7 ) . No difference was found in compact substantia nigra (22.8), dentate gyrus (26.2) and CA1, CA2, CA3 of hippocampus (>25). CONCLUSION: PPE mRNA in brain regions involved in modulation of blood pressure may be associated with the genesis of spontaneous hypertension in SHR.