| 一种从人子宫颈粘液新分离的抗菌多肽的分子特性分析 |
| |
| 引用本文： | 李明,潘小玲,王莉莉,孙艳,冯云,黄宁,吴琦,李绚,王伯瑶. 一种从人子宫颈粘液新分离的抗菌多肽的分子特性分析[J]. 四川大学学报(医学版), 2005, 36(3): 315-317 |
| |
| 作者姓名： | 李明 潘小玲 王莉莉 孙艳 冯云 黄宁 吴琦 李绚 王伯瑶 |
| |
| 作者单位： | 四川大学华西基础医学与法医学院,感染免疫研究室,成都,610041;四川大学华西第二医院,妇产科,成都,610041;四川生物制品研究所,成都,610041 |
| |
| 基金项目： | 国家自然科学基金 (批准号 3 980 0 14 6),纽约中华医学基金会 (96-681)资助 |
| |
| 摘 要： | 目的对本室从人子宫颈粘液分离纯化的1个抗菌多肽HCP-1进行分子结构特性分析。方法分离HCP-1多肽,测定其氮端氨基酸序列,根据其氮端氨基酸序列密码子设计简并引物,采用3’-RACE-PCR技术扩增HCP-1编码基因,连接到T载体进行测序。用生物软件分析测序结果。结果以筒并引物结合RACE—PCR技术成功的克隆出HCP-1全长cDNA,BLAST分析证实其序列与HMG-17分子相同,OMIGA软件分析发现其含有一个α-螺旋结构(疏水结构区)可能是其抗菌功能的基础。结论HCP-1为HMG-17,是存在于宫颈粘液抗菌的一种抗菌多肽,其中的α-螺旋结构可能是其抗菌的结构基础。
|
| 关 键 词： | 人子宫颈粘液 抗菌多肽 cDNA克隆 结构分析 |
| 修稿时间： | 2004-06-21 |
Characterization of a New Antibacterial Polypeptide Isolated from Human Cervical Mucus |
| |
| LI Ming,PAN Xiao-ling,WANG Li-li,SUN Yan,FENG Yun,HUANG Ning,WU Qi,LI Xun,WANG Bo-Yao. Characterization of a New Antibacterial Polypeptide Isolated from Human Cervical Mucus[J]. Journal of Sichuan University. Medical science edition, 2005, 36(3): 315-317 |
| |
| Authors: | LI Ming PAN Xiao-ling WANG Li-li SUN Yan FENG Yun HUANG Ning WU Qi LI Xun WANG Bo-Yao |
| |
| Affiliation: | Research Unit of Infection & Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China. |
| |
| Abstract: | OBJECTIVE: To identify a new antibactrial polypeptide HCP-1 isolated from human cervical mucus. METHODS: HCP-1 was isolated and purified from human cervical mucus, and N-terminal sequence of HCP-1 was determined. A degenerate primer was designed according to CodeHop methods, and an "anchor-oliga-dT primer" was used for the synthesis of cDNA. Using the degenerate primer and anchor-primer, cDNAs were amplified by PCR. The PCR products were cloned, sequenced, and analyzed by biological software. RESULTS: N-terminat sequence of HCP-1 was PKRKAEGDAK. The full length of HCP-1 cDNA was isolated and of which the sequence was the same as HMG-17. OMIGA software analysis indicated that this molecule contained an alpha-helix region. CONCLUSION: The new antibacterial polypeptide isolated from human cervical mucus is HMG-17. It may play a role in the human cervical mucus and the alpha-helix domain may be its antibacterial activity region. |
| |
| Keywords: | Human cervical mucus Antibacterial polypeptide cDNA cloning Structure analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
