Frequency of intake and amount of fluoride in milk for remineralisation of artificial caries on enamel and dentine: Ex vivo/in situ study |
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| Institution: | 1. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil;2. Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, SP, R. José Bonifácio, 1193, Araçatuba, SP, 16015-050, Brazil;3. School of Dentistry, Univ. Cidade de São Paulo (UNICID), R. Cesário Galeno, 448/475, São Paulo-SP, 03071-000, Brazil;4. Institute of Dentistry, Cruzeiro do Sul University, R. Galvão Bueno, 868, São Paulo-SP, 01506-000, Brazil;1. CPPAR – Animal Health Research Center – Faculdade de Ciências Agrárias e Veterinárias, UNESP, Via de acesso Prof. Paulo Donatto Castellani, s/n, CEP:14884-900 Jaboticabal, São Paulo, Brazil;2. Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74605050, Brazil;3. Universidade Camilo Castelo Branco (Unicastelo), Av. Hilário da Silva Passo 950, Descalvado, SP 13690-970, Brazil;1. Heart Institute (InCor) University of Sao Paulo Medical Scholl Hospital, Sao Paulo, Brazil;2. Instituto de Pesquisas Energéticas e Nucleares, IPEN, Sao Paulo, Brazil;3. Kagawa Nutrition University, Tokyo, Japan;4. Lipid Metabolism Laboratory, Human Nutrition Research Center on Aging at Tufts University and Tufts University School of Medicine, Boston, MA, USA |
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| Abstract: | ObjectivesThis study analysed the effect of frequency of intake and amount of fluoride in milk on the remineralisation of artificial enamel and dentine caries lesions ex vivo/in situ.Materials and methodsPre-demineralised bovine enamel and dentine slabs were randomly allocated into 5 groups and fixed in removable appliances used by subjects for 7 days in each phase. Each treatment comprised milk containing 2.5 ppm fluoride daily (T1), or every other day (T2), 5.0 ppm F daily (T3), or every other day (T4) or no treatment (T5).ResultsEnamel alterations were quantified by surface hardness recovery (%SHR) and transversal microradiography (TMR), and in dentine by TMR only. Data were analysed by ANOVA and Tukey’s test (p < 0.05). For enamel, the highest %SHR was found for T1 and T3 compared to control, without significant differences between them. All groups showed positive values of ΔΔZ ? T1 (247.3 ± 198.5); T2 (110.9 ± 303.2); T3 (226.0 ± 299.2); T5 (5.0 ± 288.0), except T4 (?274.5 ± 407.3). For dentine, the only group that presented remineralisation was T2 (350.0 ± 657.5).ConclusionsFluoridated milk daily seems to have higher remineralising effect on enamel than its use every other day. Dentine, does not seem to benefit from daily use of fluoridated milk. |
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| Keywords: | Enamel Dentine Fluoride Milk Remineralisation Caries |
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